Prasertsee Teerarat, Chokesajjawatee Nipa, Santiyanont Pannita, Chuammitri Phongsakorn, Deeudom Manu, Tadee Pakpoom, Patchanee Prapas
Graduate Program in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Muang, Thailand.
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
Zoonoses Public Health. 2019 May;66(3):301-309. doi: 10.1111/zph.12565. Epub 2019 Feb 5.
Human salmonellosis is a major public health problem worldwide. Infections can pass to humans by contact with contaminated substances in the food chain. This study aimed to determine the prevalence and contamination levels of Salmonella isolated from pork, chicken and beef sold in different types of retail stores in Chiang Mai and Lamphun provinces and to investigate the genetic relatedness among Salmonella isolates in food chains in that area. A total of 360 meat samples from supermarkets, mini-grocery stores and fresh markets were obtained. Salmonella Rissen and S. Weltevreden were found in all meat sample types and in human cases. The overall prevalence of Salmonella in the chicken, pork and beef samples was 34.17%, 32.50% and 3.33%, respectively. Quantitatively, Salmonella contamination was highest in pork (1.24 log MPN/g), followed by chicken (1.08 log MPN/g), and beef (0.75 log MPN/g). The highest frequency of Salmonella contamination was found at the fresh markets (85.71%), whereas the highest quantity of contamination level was from mini-grocery stores (1.27 log MPN/g). The rep-PCR analysis results revealed that some of the Salmonella from meat samples and human cases were identical clones.
人类沙门氏菌病是全球主要的公共卫生问题。感染可通过接触食物链中受污染的物质传播给人类。本研究旨在确定从清迈和南奔府不同类型零售商店销售的猪肉、鸡肉和牛肉中分离出的沙门氏菌的流行率和污染水平，并调查该地区食物链中沙门氏菌分离株之间的遗传相关性。共采集了来自超市、小杂货店和生鲜市场的360份肉类样本。在所有肉类样本类型以及人类病例中均发现了里森沙门氏菌和韦尔泰弗里登沙门氏菌。鸡肉、猪肉和牛肉样本中沙门氏菌的总体流行率分别为34.17%、32.50%和3.33%。从数量上看，猪肉中的沙门氏菌污染最高（1.24 log MPN/g），其次是鸡肉（1.08 log MPN/g），牛肉（0.75 log MPN/g）。沙门氏菌污染频率最高的是生鲜市场（85.71%），而污染水平最高的数量来自小杂货店（1.27 log MPN/g）。重复聚合酶链反应分析结果显示，肉类样本和人类病例中的一些沙门氏菌是相同的克隆株。
