Ahmad Hajam Younis, Rai Seema, Basheer Muddasir, Ghosh Hindole, Singh Srishti
Pak J Biol Sci. 2018 Jan;21(9):423-431. doi: 10.3923/pjbs.2018.423.431.
Hyperglycemia is a representative hallmark and risk factor for diabetes and is closely linked to diabetes associated complications. The aim of the present study was to evaluate the therapeutic potential of exogenous melatonin against the streptozotocin induced pancreatic damages in rats.
Streptozotocin was injected for consecutive 6 days. Diabetes was confirmed by blood glucose measurement after 72 h and on 7th day after injection. Animals having blood glucose level above 250 mg dL-1 were considered as diabetic and were administered exogenous melatonin for 4 weeks. Animals were euthanized after last dose, pancreas were dissected out, weighed and fixed in Bouin's fixative for histology and further tissues were kept at -20°C for biochemistry.
Diabetic rats displayed significant increase in lipid peroxidation, but pancreatic weight index, antioxidant system (GSH, SOD and CAT) showed decrease. Melatonin treatment to diabetic rats restored the alteration in physiological and biochemical markers. Results were supported by the histopathological observations, STZ treated pancreas showed damage in islets of langerhans, while as melatonin treated diabetic rats recovered the cellular architecture which inturn normalize the function of the pancreas.
Therefore, melatonin might be considered as a molecule to protect the pancreatic damages.
高血糖是糖尿病的典型特征和危险因素，与糖尿病相关并发症密切相关。本研究的目的是评估外源性褪黑素对链脲佐菌素诱导的大鼠胰腺损伤的治疗潜力。
连续6天注射链脲佐菌素。注射后72小时及第7天通过测量血糖确认糖尿病。血糖水平高于250mg/dL-1的动物被视为糖尿病动物，并给予外源性褪黑素4周。最后一剂后对动物实施安乐死，取出胰腺，称重并固定于布因氏固定液中用于组织学检查，其余组织保存于-20°C用于生化分析。
糖尿病大鼠脂质过氧化显著增加，但胰腺重量指数、抗氧化系统（谷胱甘肽、超氧化物歧化酶和过氧化氢酶）降低。对糖尿病大鼠进行褪黑素治疗可恢复生理和生化指标的改变。组织病理学观察结果支持了该结果，链脲佐菌素处理的胰腺显示胰岛损伤，而褪黑素处理的糖尿病大鼠恢复了细胞结构，进而使胰腺功能正常化。
因此，褪黑素可被视为一种保护胰腺损伤的分子。
