Division of Nutritional Biochemistry, National Institute of Fundamental Studies, Hantana Road, Kandy, Sri Lanka; Postgraduate Institute of Science, University of Peradeniya, Sri Lanka.
Division of Nutritional Biochemistry, National Institute of Fundamental Studies, Hantana Road, Kandy, Sri Lanka.
Food Chem. 2019 Mar 1;275:265-272. doi: 10.1016/j.foodchem.2018.09.114. Epub 2018 Sep 19.
Glucose oxidase (GOD) is an enzyme widely used in glucose monitoring systems owing to its high specificity towards glucose. However, in our previous work maltose was found to show significant interaction with GOD and based on this observation, a novel microplate-based method was developed to assess α-amylase inhibitory activity (GOD method). Concerns regarding the interaction of GOD with maltose has limited the widespread use of the GOD method in assessing α-amylase activity. The present paper provides answers to concerns regarding the interaction of GOD with maltose using HPLC studies and application of the GOD method in assessing α-amylase activity. According to the results, the newly developed GOD method can be considered as a well-suited method for the determination of α-amylase activity and as an easy method to do kinetic studies compared to other available methods.
葡萄糖氧化酶（GOD）是一种广泛应用于葡萄糖监测系统的酶，因为它对葡萄糖具有很高的特异性。然而，在我们之前的工作中发现麦芽糖与 GOD 有显著的相互作用，基于这一观察结果，我们开发了一种新的基于微孔板的方法来评估α-淀粉酶抑制活性（GOD 法）。由于担心 GOD 与麦芽糖的相互作用，GOD 法在评估α-淀粉酶活性方面的广泛应用受到了限制。本文通过 HPLC 研究和 GOD 法在评估α-淀粉酶活性中的应用，回答了关于 GOD 与麦芽糖相互作用的问题。结果表明，与其他可用方法相比，新开发的 GOD 法可以作为一种测定α-淀粉酶活性的合适方法，并且是一种进行动力学研究的简便方法。
