Key Laboratory of Natural Product and Functional Food of Jiangxi, Nanchang 330045, China.
Food Funct. 2019 Feb 20;10(2):1109-1122. doi: 10.1039/c8fo02312c.
In this study, the regulative effects of ovotransferrin (OVT) on immunomodulatory function and intestinal microbial dysbiosis in a mouse model injected with cyclophosphamide (CP) were investigated. The immunomodulatory effect of OVT was determined by enzyme-linked immune sorbent assay (ELISA). Gut microbial composition was determined by high-throughput sequencing of the V3-V4 region of the 16S rDNA gene. The changes in the relative abundance of the dominant microbiota were analyzed at different taxonomic levels. The results showed that OVT alleviated the immune dysfunction caused by CP. OVT improved the spleen and thymus indices and enhanced the secretion of tumor necrosis factor alpha (TNF-α), interleukin-10 (IL-10), and immunoglobulin A (IgA). In addition, OVT increased the indexes of Shannon and Simpson, suggesting the enhancement of the diversity and richness of intestinal microflora. The relative abundance of Lachnospiraceae_NK4A136_group was also increased. However, the relative abundance of Helicobacter and Desulfovibrio was significantly decreased. These results indicated that OVT, a food-derived functional component, has effects on immune regulation in the organism and ameliorates the gut microbiota disorders induced by CP, which provides a potential therapeutic utilization of avian eggs by targeting the gut microbiome.
在这项研究中，研究了卵转铁蛋白（OVT）对环磷酰胺（CP）注射小鼠模型免疫调节功能和肠道微生物失调的调节作用。通过酶联免疫吸附试验（ELISA）测定 OVT 的免疫调节作用。通过高通量测序 V3-V4 区 16S rDNA 基因测定肠道微生物组成。在不同分类水平上分析了主要微生物群落相对丰度的变化。结果表明，OVT 减轻了 CP 引起的免疫功能障碍。OVT 提高了脾脏和胸腺指数，增强了肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和免疫球蛋白 A（IgA）的分泌。此外，OVT 增加了 Shannon 和 Simpson 指数，表明肠道微生物群落的多样性和丰富度得到增强。Lachnospiraceae_NK4A136_group 的相对丰度也增加了。然而， Helicobacter 和 Desulfovibrio 的相对丰度明显下降。这些结果表明，OVT 作为一种食物来源的功能成分，对机体的免疫调节具有作用，并改善 CP 诱导的肠道微生物失调，为通过靶向肠道微生物组利用禽蛋提供了一种潜在的治疗用途。
