Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Riga, Latvia.
Department of Agricultural, Food and Environmental Sciences, Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, Italy.
Yeast. 2019 May;36(5):375-379. doi: 10.1002/yea.3382. Epub 2019 Mar 5.
Yeast cells are able to transition into a state of anhydrobiosis (temporary reversible suspension of metabolism) under conditions of desiccation. One of the most efficient approaches for understanding the mechanisms underlying resistance to dehydration-rehydration is to identify yeasts, which are stable under such treatments, and compare them with moderately resistant species and strains. In the current study, we investigated the resistance to dehydration-rehydration of six psychrotolerant yeast strains belonging to two species. All studied strains of Solicoccozyma terricola and Naganishia albida were found to be highly resistant to dehydration-rehydration. The viability of S. terricola strains was close to 100%. Such results have not been previously reported in studies of anhydrobiosis in yeasts. The plasma membrane changes, revealed by determining its permeability under various rehydration conditions, were also surprisingly minimal. Thus, the high level of resistance of psychrotolerant yeast strains might be related to the chemical composition and molecular organisation of their plasma membranes. Aside from plasma membrane characteristics, other important factors may also influence the maintenance of yeast cell viability under conditions of dehydration-rehydration.
在干燥条件下，酵母细胞能够进入休眠状态（代谢暂时可逆暂停）。了解抗干燥-复水机制的最有效方法之一是鉴定在这种处理下稳定的酵母，并将其与中度抗性的物种和菌株进行比较。在本研究中，我们研究了属于两个种的六种耐冷酵母菌株对干燥-复水的抗性。发现所有研究的 Sollicoccozyma terricola 和 Naganishia albida 菌株对干燥-复水具有高度抗性。S. terricola 菌株的存活率接近 100%。在以前对酵母休眠的研究中，没有报道过这样的结果。通过在各种复水条件下测定其通透性来揭示的质膜变化也非常小。因此，耐冷酵母菌株的高抗性可能与其质膜的化学组成和分子组织有关。除了质膜特性外，其他重要因素也可能影响酵母细胞在干燥-复水条件下的存活。
