Kaplan S L, Hawkins E P, Inzana T J, Patrick C C, Mason E O
Myers Black Section of Pediatric Infectious Diseases, Texas Children's Hospital, Houston 77030.
Microb Pathog. 1988 Jul;5(1):55-62. doi: 10.1016/0882-4010(88)90081-2.
The mechanism(s) by which the lipopolysaccharide (LPS) of Haemophilus influenzae type b may contribute to the virulence of this organism is unclear. Purified LPS of Haemophilus influenzae type b or phosphate buffered saline was administered intranasally to infant rats prior to the intranasal instillation of approximately 2-20 x 10(6) cfu of Hib two or three times per day for three consecutive days. The preadministration of 2.0 micrograms Hib LPS resulted in a significantly greater incidence of bacteremia (P = 0.0006) than PBS 30 min after the completion of the intranasal inoculation. Four days following completion of intranasal Hib inoculation the incidence of bacteremia was greater (P = 0.017) in the animals pretreated with LPS at 2.0 micrograms compared to the PBS pretreated animals. Preadministration of 0.2 micrograms LPS had no effect on the incidence of bacteremia or meningitis. There were no differences in the histology of the nasal cavities or turbinates of infant rats inoculated intranasally only with LPS or PBS. There were no differences in the frequency or density of bacteremia following intranasal administration of LPS from either Hib or E. coli. Although the mechanism is unknown, our findings suggest that the LPS of Hib may contribute to the ability of H. influenzae type b to invade the nasal mucosa in this infant rat model.
b型流感嗜血杆菌的脂多糖（LPS）可能促成该生物体毒力的机制尚不清楚。在连续三天每天两到三次经鼻滴注约2 - 20×10⁶cfu的b型流感嗜血杆菌（Hib）之前，将纯化的b型流感嗜血杆菌LPS或磷酸盐缓冲盐水经鼻给予幼鼠。在经鼻接种完成30分钟后，预先给予2.0微克Hib LPS导致菌血症的发生率显著高于PBS（P = 0.0006）。在经鼻接种Hib完成四天后，与预先用PBS处理的动物相比，用2.0微克LPS预处理的动物中菌血症的发生率更高（P = 0.017）。预先给予0.2微克LPS对菌血症或脑膜炎的发生率没有影响。仅经鼻接种LPS或PBS的幼鼠鼻腔或鼻甲的组织学没有差异。经鼻给予来自Hib或大肠杆菌的LPS后，菌血症的频率或密度没有差异。尽管机制尚不清楚，但我们的研究结果表明，在这个幼鼠模型中，Hib的LPS可能有助于b型流感嗜血杆菌侵袭鼻黏膜的能力。
