Kettunen Mikko I
A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
Methods Mol Biol. 2019;1928:409-426. doi: 10.1007/978-1-4939-9027-6_21.
Hyperpolarized magnetic resonance imaging (MRI) can be used to detect real-time in vivo tumor metabolism. Dissolution dynamic nuclear polarization method increases polarization of C-labeled molecules, typically [1-C]pyruvate, which can be injected into an animal during MRI scanning. Increased polarization leads to a higher observed signal, which allows for the detection and imaging of the transfer of C-label between the injected marker molecule, pyruvate, and its metabolic products, most importantly lactate. This information can be used to assess the metabolic status of the tumor, for example, during therapy. Here, the basic methodology and data analysis for a preclinical hyperpolarized pyruvate experiment are described.
超极化磁共振成像（MRI）可用于检测体内实时肿瘤代谢。溶解动态核极化方法可增加碳标记分子（通常是[1-¹³C]丙酮酸）的极化，该分子可在MRI扫描期间注入动物体内。极化增加会导致更高的观测信号，从而能够检测和成像注入的标记分子丙酮酸与其代谢产物（最重要的是乳酸）之间的¹³C标记转移。这些信息可用于评估肿瘤的代谢状态，例如在治疗期间。本文描述了临床前超极化丙酮酸实验的基本方法和数据分析。
