Branch Bioanalytics and Bioprocesses, Department Cellular Biotechnology & Biochips , Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13 , 14476 Potsdam-Golm , Germany.
NanoBioTechnology Group, Department of Biotechnology, Faculty of Bioscience Engineering , Ghent University , 9000 Ghent , Belgium.
Langmuir. 2019 Jul 2;35(26):8565-8573. doi: 10.1021/acs.langmuir.8b04328. Epub 2019 Feb 15.
Here we address research directions and trends developed following novel concepts in 2D/3D self-assembled polymer structures established in the department led by Helmuth Möhwald. These functional structures made of hybrids of polymer multilayers, lipids, and nanoparticles stimulated research in the design of the cellular microenvironment. The composition of the extracellular matrix (ECM) and dynamics of biofactor presentation in the ECM can be recapitulated by the hybrids. Proteins serve as models for protein-based biofactors such as growth factors, cytokines, hormones, and so forth. A fundamental understanding of complex intermolecular interactions and approaches developed for the externally IR-light-triggered release offers a powerful tool for controlling the biofactor presentation. Pure protein beads made via a mild templating on vaterite CaCO crystals can mimic cellular organelles in terms of the compartmentalization of active proteins. We believe that an integration of the approaches developed and described here offers a strong tool for engineering and mimicking both extra- and intracellular microenvironments.
在这里，我们将讨论在 Helmuth Möhwald 领导的部门中建立的 2D/3D 自组装聚合物结构的新概念所带来的研究方向和趋势。这些由聚合物多层、脂质和纳米粒子的混合物组成的功能性结构激发了对细胞微环境设计的研究。细胞外基质 (ECM) 的组成和生物因子在 ECM 中的呈现动力学可以通过混合物来再现。蛋白质可以作为基于蛋白质的生物因子（如生长因子、细胞因子、激素等）的模型。对复杂的分子间相互作用的基本理解以及为外部红外光触发释放而开发的方法为控制生物因子呈现提供了强大的工具。通过在水碳酸钙晶体上进行温和的模板化，可以制备出纯蛋白质珠，这些蛋白质珠可以在活性蛋白质的分隔化方面模拟细胞细胞器。我们相信，这里所描述的方法的整合为工程和模拟细胞外和细胞内微环境提供了强有力的工具。
