Akiyama Kentaro, Chen Chider, Gronthos Stan, Shi Songtao
Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, USA.
Methods Mol Biol. 2012;887:111-21. doi: 10.1007/978-1-61779-860-3_11.
Recently, a variety of mesenchymal stem cells (MSCs), including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and mesenchymal stem cells derived from human gingival, were isolated from orofacial and dental tissues. However, it is unknown whether these orofacial stem cells are derived from mesoderm or neural crest cell. In order to encourage orofacial MSC investigation, we provide detailed protocols for assessing lineage -differentiation of orofacial MSCs.
最近，从口腔颌面部和牙齿组织中分离出了多种间充质干细胞（MSC），包括牙髓干细胞、人脱落乳牙干细胞、根尖乳头干细胞、牙周膜干细胞以及来源于人牙龈的间充质干细胞。然而，这些口腔颌面部干细胞是否来源于中胚层或神经嵴细胞尚不清楚。为了促进口腔颌面部间充质干细胞的研究，我们提供了评估口腔颌面部间充质干细胞谱系分化的详细方案。
