Karched Maribasappa, Ali Dena, Ngo Hien
Oral Microbiology Research Laboratory, Department of Bioclinical Sciences, Faculty of Dentistry, Kuwait University.
Department of General Dental Practice, Faculty of Dentistry, Kuwait University.
J Oral Sci. 2019 Mar 28;61(1):19-24. doi: 10.2334/josnusd.17-0366. Epub 2019 Feb 6.
Minimally invasive treatment protocols may leave a residual layer of carious dentin, which requires treatment for the inhibition of bacterial growth beneath restorations. We aimed to examine the in vivo effect of silver diammine fluoride (SDF) and SDF + potassium iodide (KI) application on bacteria present in deep carious lesions. We studied the in vivo efficacy in five patients, each of which had five carious lesions. Dentin samples taken before and after treatment were subjected to microbial analyses. Following treatment with SDF, the median colony-forming unit (CFU) counts per mg of dentin reduced from 9 × 10 to 1.6 × 10 (P < 0.05), and following that with SDF + KI, the counts decreased from 2.9 × 10 to 9.2 × 10 (P < 0.05). The use of chlorhexidine gluconate (CHX) reduced CFU counts from 1.1 × 10 to 4.8 × 10 (P < 0.05). In four of the five patients, no CFUs were found on mitis salivarius-bacitracin agar with respect to SDF or SDF + KI application. For CHX, the median CFU count before treatment was 1.6 × 10 and that after treatment was 1.1 × 10. SDF completely inhibited mutans streptococci growth in four of the five patients, while the growth of anaerobes was not completely inhibited.
微创治疗方案可能会留下一层龋坏牙本质，这需要进行治疗以抑制修复体下方细菌的生长。我们旨在研究应用氨硝酸银（SDF）和SDF + 碘化钾（KI）对深龋病变中存在的细菌的体内作用。我们对五名患者进行了体内疗效研究，每名患者有五个龋损。对治疗前后采集的牙本质样本进行微生物分析。用SDF治疗后，每毫克牙本质的菌落形成单位（CFU）中位数从9×10降至1.6×10（P < 0.05），用SDF + KI治疗后，CFU计数从2.9×10降至9.2×10（P < 0.05）。使用葡萄糖酸氯己定（CHX）可使CFU计数从1.1×10降至4.8×10（P < 0.05）。在五名患者中的四名患者中，应用SDF或SDF + KI后，在轻唾-杆菌肽琼脂上未发现CFU。对于CHX，治疗前CFU计数中位数为1.6×10，治疗后为1.1×10。SDF在五名患者中的四名患者中完全抑制了变形链球菌的生长，而厌氧菌的生长未被完全抑制。
