Fiorentini C, Arancia G, Caprioli A, Falbo V, Ruggeri F M, Donelli G
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Toxicon. 1988;26(11):1047-56. doi: 10.1016/0041-0101(88)90203-6.
The effect of the cytotoxic necrotizing factor of Escherichia coli on HEp-2 cells was studied by fluorescence and scanning electron microscopy. This cytotoxin, known for inducing the formation of giant multinucleated cells in several cell lines, caused changes in actin and tubulin organization. The presence of membrane ruffles at the cell border and of numerous thick bundles of actin crossing the cell body, suggests that the factor promotes cell spreading; this probably interferes with cytokinesis, ultimately leading to the formation of very large flattened multinucleated cells. Moreover, the nuclear segmentation observed in treated cells seems to be associated with a rearrangement of actin in the perinuclear region and with the presence of tubulin bundles in proximity to nuclear clefts. Although the primary target is still unknown, these findings suggest that the cytoskeleton is affected accounting for the multinucleation process induced by the factor.
通过荧光显微镜和扫描电子显微镜研究了大肠杆菌细胞毒素坏死因子对人喉表皮样癌细胞（HEp-2细胞）的作用。这种细胞毒素以在多种细胞系中诱导形成巨大的多核细胞而闻名，它会引起肌动蛋白和微管蛋白组织的变化。在细胞边界处存在膜皱褶以及许多穿过细胞体的粗大肌动蛋白束，这表明该因子促进细胞铺展；这可能会干扰胞质分裂，最终导致形成非常大的扁平多核细胞。此外，在处理过的细胞中观察到的核分裂似乎与核周区域肌动蛋白的重排以及核裂附近微管蛋白束的存在有关。尽管主要靶点仍然未知，但这些发现表明细胞骨架受到影响是该因子诱导多核化过程的原因。
