Kulkarni Ruta, Tiraki Divya, Wani Dileep, Mishra Akhilesh C, Arankalle Vidya A
Department of Communicable Diseases, Interactive Research School for Health Affairs, Bharati Vidyapeeth (Deemed To Be University), Pune, India.
Department of Immunohaematology and Blood Transfusion, Bharati Medical College and Research Center, Bharati Vidyapeeth (Deemed To Be University), Pune, India.
Transfusion. 2019 Feb;59(2):458-462. doi: 10.1111/trf.15007. Epub 2018 Nov 10.
Dengue, a mosquito-borne viral disease, is endemic in >125 countries worldwide. The threat of blood-borne transmission of dengue virus (DENV) has been documented.
This study was conducted to assess the potential magnitude of transfusion-associated dengue, by determination of DENV seromarkers in blood donations from Pune, India, during two dengue seasons (2016 and 2017). These included DENV nonstructural protein 1 (NS1), anti-DENV immunoglobulin (Ig) M, anti-DENV IgG (enzyme-linked immunosorbent assay), and DENV RNA (reverse transcription-polymerase chain reaction).
NS1 (IgM) reactivity was 1 of 209, 0.48% (11/209, 5.3%) in 2016 and 2 of 311, 0.64% (20/311, 6.4%) in 2017. Of the 34 NS1/IgM reactives, 1 NS1-reactive donor and 10 IgM-reactive donors exhibited evidence of secondary infection. DENV RNA was not detected in any of the 34 NS1/IgM reactives. Among the NS1/IgM negatives, anti-DENV IgG reactivity was high in 2016 (75%) and further increased in 2017 (87%, p = 0.002).
Although RNA negative, detection of 34 NS1/IgM-reactive donations, of which 11 had evidence of secondary infection, suggests the need for further evaluation on the basis of potential risk to recipients of either dengue transmission or increased risk of secondary infection. These would include multicenter studies followed by cost-benefit analyses.
登革热是一种由蚊子传播的病毒性疾病，在全球超过125个国家流行。已有文献记载登革热病毒（DENV）通过血液传播的威胁。
本研究旨在通过测定印度浦那在两个登革热季节（2016年和2017年）献血者中的DENV血清标志物，评估输血相关登革热的潜在严重程度。这些标志物包括DENV非结构蛋白1（NS1）、抗DENV免疫球蛋白（Ig）M、抗DENV IgG（酶联免疫吸附测定）和DENV RNA（逆转录-聚合酶链反应）。
NS1（IgM）反应性在2016年为209份中的1份，0.48%（11/209，5.3%），在2017年为311份中的2份，0.64%（20/311，6.4%）。在34份NS1/IgM反应性样本中，1名NS1反应性献血者和10名IgM反应性献血者表现出二次感染的证据。在34份NS1/IgM反应性样本中均未检测到DENV RNA。在NS1/IgM阴性样本中，抗DENV IgG反应性在2016年较高（75%），在2017年进一步升高（87%，p = 0.002）。
尽管RNA为阴性，但检测到34份NS1/IgM反应性献血样本，其中11份有二次感染的证据，这表明需要根据对受血者登革热传播的潜在风险或二次感染风险增加进行进一步评估。这些评估将包括多中心研究，随后进行成本效益分析。
