Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College, Chengdu, China.
Key Laboratory of Transfusion Adverse Reactions, CAMS, Chengdu, China.
BMC Infect Dis. 2021 Jan 22;21(1):104. doi: 10.1186/s12879-021-05810-8.
Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies.
Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed.
Over the study period, 2254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P = 0.001), education (P < 0.001), and ethnicity (P = 0.026).
The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
登革热病毒（DENV）感染在中国南方越来越常见，可通过输血传播，但目前该地区并非所有献血者都进行 DENV 筛查。我们评估了云南西双版纳血站献血者中 DENV 的流行情况，以支持制定 DENV 筛查策略。
2019 年 6 月至 8 月间随机采集血样。采用酶联免疫吸附试验（ELISA）检测抗 DENV IgG 和 IgM。然后，使用实时聚合酶链反应（RT-PCR）检测所有反应性样本和部分随机选择的非反应性样本中的 DENV RNA。RT-PCR 后，使用胶体金法检测可溶性非结构蛋白 1（NS1）。还收集并评估了献血者的人口统计学数据。
在研究期间，共采集 2254 份献血者样本进行 ELISA 检测抗 DENV IgG 和 IgM，发现 598 份抗 DENV IgG 和/或 IgM 反应性样本，血清学流行率为 26.53%。其中 26 份为 RT-PCR 阳性和/或 NS1 阳性。职业（P=0.001）、教育程度（P<0.001）和种族（P=0.026）差异与 DENV 流行率显著相关。
西双版纳血站的 DENV 流行率高于大多数已实施 DENV 献血者筛查的其他血站。本研究提供了有关 DENV 流行情况的第一手数据，并为临床应用开发了筛查策略。
