Center for Dengue Fever Control and Research, Kaohsiung Medical University, Kaohsiung City, Taiwan.
Tropical Medicine Center, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan.
PLoS One. 2018 Oct 8;13(10):e0205248. doi: 10.1371/journal.pone.0205248. eCollection 2018.
To determine the prevalence of asymptomatic dengue virus-infected blood donors during the largest dengue outbreak in Taiwan history occurred in 2015, we examined the evidence of dengue virus (DENV) infection by the detection of DENV RNA genome using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR), DENV NS1 antigen using rapid diagnosis test (RDT) and anti-dengue antibody using IgM/IgG capture enzyme-linked immunosorbent assay (capture ELISA) and RDT in eight thousand serum samples from blood donations to the blood centers of the Taiwan Blood Services Foundation (TBSF) in Kaohsiung City and Tainan City during the largest dengue outbreak in Taiwan history occurred in 2015. Only one serum sample was positive for DENV RNA detection by using dengue-specific real-time RT-PCR, the virus was DENV-2 determined by serotype-specific real-time RT-PCR and sequencing, and the DENVs in the serum were confirmed as being infectious by a plaque assay. The recipient of this blood did not develop any dengue fever symptom on follow-up. None of the samples was NS1 RDT-reactive. Seventeen IgM-positive samples were identified. There was a low prevalence of asymptomatic confirmed or probable DENV-infected blood donors in our study (0.013% and 0.21%, respectively), and no symptomatic transfusion-transmitted dengue (TT dengue) was developed during the largest dengue outbreak in Taiwan history in highly endemic areas and periods.
为了确定 2015 年台湾历史上最大的登革热疫情期间无症状登革病毒感染献血者的流行率，我们通过实时逆转录聚合酶链反应（real-time RT-PCR）检测登革病毒（DENV）RNA 基因组、使用快速诊断检测（RDT）检测 DENV NS1 抗原和使用 IgM/IgG 捕获酶联免疫吸附试验（capture ELISA）和 RDT 检测高雄市和台南市台湾血液基金会（TBSF）血液中心的 8000 份献血者血清样本，以寻找登革病毒感染的证据。在 2015 年台湾历史上最大的登革热疫情期间，只有一份血清样本通过使用登革热特异性实时 RT-PCR 检测到 DENV RNA 呈阳性，通过血清型特异性实时 RT-PCR 和测序确定该病毒为 DENV-2，通过蚀斑试验证实血清中的 DENVs 具有感染性。接受该血液的患者在随访中未出现任何登革热症状。没有样本对 NS1 RDT 有反应。确定了 17 份 IgM 阳性样本。在我们的研究中，无症状确诊或可能感染 DENV 的献血者的流行率较低（分别为 0.013%和 0.21%），在高度流行地区和流行期间，没有发生症状性输血传播登革热（TT 登革热）。
