Lee H B
Division of Applied Bioscience and Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Korea. This study was funded by NRF (2010-0012153) and in part supported by the Technology Development Program (111095-3) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
Plant Dis. 2012 Dec;96(12):1827. doi: 10.1094/PDIS-08-12-0754-PDN.
Lanceleaf coreopsis (Coreopsis lanceolata L.) is a plant species of the genus Coreopsis in Asteraceae native to the USA. This plant is a bushy perennial species with finely cut foliage and showy round flowers and is increasing as plants used in landscaping in the world. The invasive plant is also planted very commonly along roadsides in Korea. In late October 2011 and August 2012, signs and symptoms of a powdery mildew disease were observed on lanceleaf coreopsis in several land areas near Gwangju-river, Gwangju, Korea. Symptoms included typical white superficial mycelia. The conidia of the powdery mildew fungus occurred on adaxial and abaxial surfaces. Chasmothecia were not observed. Single conidia formed terminally on conidiophores. Conidial morphology was subcylindrical to oblong. Dimension of conidia was 23.6 to 41.4 (avg. 35.1) μm long × 11.3 to 18.2 (avg. 14.8) μm wide. Conidiophores were composed of five to six (up to seven) cells, ranged from 45.7 to 131.2 (avg. 98.1) μm long × 8.2 to 11.1 (avg. 8.3) μm wide with foot-cells straight to slightly flexuous. Oidium anamorph of this fungus matched that of E. arcuata U. Braun, Heluta and S. Takam. described by Pastircakova et al. (3). From extracted genomic DNA, the rDNA ITS was amplified with ITS1F (5'-CTTGGTCATTTAGAGGAAGT-3') and LR5F (5'-GCTATCCTGAGGGAAAC-3') primer set. The rDNA ITS homology of the fungus (EML-CDPW1, GenBank Accession No. JX485650) showed 100% (590/590) identity value with E. arcuata (GenBank Accession No. AB252459). The identification of the fungus as E. arcuata was based on morphological data combined with the results of sequence analysis. Until recently, E. arcuata has been known to widespread on Carpinus species of the family Betulaceae including European hornbeam (C. betulus L.) and Chonowski's hornbeam (C. tschonoskii Maxim.) in Asia and Europe since Braun et al. first reported it as a new species in 2006 (1). In Korea, Podosphaera fusca (= Sphaerotheca fusca) and P. fuliginea (= S. fuliginea) were reported to cause powdery mildews on Coreopsis lanceolata. E. cichoracearum (= Golovinomyces cichoracearum), Leveillula taurica, P. fusca (= S. fusca), and Oidium spp. have been reported on Coreopsis spp. in the world (3). To our knowledge, this is the first report of powdery mildew caused by Oidium anamorph of E. arcuata on lanceleaf coreopsis (C. lanceolata) in Korea or elsewhere in the world. References: (1) U. Braun et al. Mycol. Prog. 5:139, 2006. (2) D. F. Farr and A. Y. Rossman. Fungal Databases, Systematic Mycology and Microbiology Laboratory, ARS, USDA. http://nt.ars-grin.gov/fungaldatabases/ , 2012. (3) K. Pastircakova et al. J. Phytopathol. 156:597, 2008.
柳叶金鸡菊（Coreopsis lanceolata L.）是菊科金鸡菊属的一种植物，原产于美国。这种植物是一种多年生丛生植物，叶片细碎，花朵呈艳丽的圆形，在世界各地作为园林植物的使用量正在增加。这种入侵植物在韩国的路边也很常见。2011年10月下旬和2012年8月，在韩国光州广域市光州河附近的几个陆地区域，柳叶金鸡菊上观察到白粉病的症状和体征。症状包括典型的白色表面菌丝体。白粉病菌的分生孢子出现在叶片的正面和背面。未观察到闭囊壳。单个分生孢子在分生孢子梗顶端形成。分生孢子形态为近圆柱形至长方形。分生孢子大小为23.6至41.4（平均35.1）μm长×11.3至18.2（平均14.8）μm宽。分生孢子梗由五至六个（最多七个）细胞组成，长45.7至131.2（平均98.1）μm×宽8.2至11.1（平均8.3）μm，基部细胞直或稍弯曲。这种真菌的粉孢型无性态与Pastircakova等人（3）描述的E. arcuata U. Braun、Heluta和S. Takam的相符。从提取的基因组DNA中，用ITS1F（5'-CTTGGTCATTTAGAGGAAGT-3'）和LR5F（5'-GCTATCCTGAGGGAAAC-3'）引物对扩增rDNA ITS。该真菌（EML-CDPW1，GenBank登录号JX485650）的rDNA ITS同源性与E. arcuata（GenBank登录号AB252459）显示100%（590/590）的同一性值。基于形态学数据和序列分析结果，将该真菌鉴定为E. arcuata。直到最近，自Braun等人于2006年首次将其报道为新物种以来，已知E. arcuata在亚洲和欧洲的桦木科鹅耳枥属物种上广泛分布，包括欧洲鹅耳枥（C. betulus L.）和朝鲜鹅耳枥（C. tschonoskii Maxim.）。在韩国，据报道，球针壳属（Podosphaera fusca = Sphaerotheca fusca）和暗球针壳（P. fuliginea = S. fuliginea）会引起柳叶金鸡菊上的白粉病。在世界范围内，已报道菊科植物上有瓜白粉菌（E. cichoracearum = Golovinomyces cichoracearum）、牛舌草内丝白粉菌（Leveillula taurica）、球针壳属（Podosphaera fusca = Sphaerotheca fusca）和粉孢属（Oidium spp.）。据我们所知，这是韩国或世界其他地方首次报道由E. arcuata的粉孢型无性态引起的柳叶金鸡菊（C. lanceolata）白粉病。参考文献：（1）U. Braun等人，《真菌进展》5:139，2006年。（②）D. F. Farr和A. Y. Rossman，《真菌数据库》，系统真菌学和微生物学实验室，美国农业部农业研究局，http://nt.ars-grin.gov/fungaldatabases/，2012年。（3）K. Pastircakova等人，《植物病理学杂志》156:597，2008年。
