Chang C -C, Locy R D, Smeda R, Sahi S V, Singh N K
Department of Botany and Microbiology, Auburn University, Room 101, Rouse Life Sciences Building, 36849, Auburn, AL, USA.
Department of Biology, Alabama State University, 36101, Montgomery, AL, USA.
Plant Cell Rep. 1997 Apr;16(7):495-502. doi: 10.1007/BF01092773.
Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l NaCl. Both salt-adapted cultures maintained in 20 g l NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O evolution and[CO] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.
光合自养烟草（Nicotiana tabacum var. Wisconsin 38）细胞培养物逐渐适应在含有通常具有抑制作用的20 g/L NaCl浓度的培养基中生长。在20 g/L NaCl中维持的盐适应培养物（P20）和未适应盐的培养物（P0）在叶绿体形态和基于干重的生长特性方面表现出相似性，但与P0细胞相比，P20细胞在鲜重基础上生长减缓。与P0细胞相比，P20细胞内蔗糖水平显著更高，而P0细胞中的淀粉水平显著高于P20细胞。P20和P0细胞中细胞内和细胞外还原糖以及叶绿素的积累程度相同，但P20细胞中的积累延迟了约13天。P20细胞中O₂释放和[CO₂]固定比P0细胞更能抵抗NaCl的抑制。然而，尽管与P20叶绿体相比，在P0中观察到每毫克叶绿素基础上Rubisco水平更高，但在P20和P0细胞之间未显示类囊体膜蛋白丰度有显著变化。
