半连续培养条件下未驯化和 NaCl 驯化烟草细胞的生长产率和维持系数。
Growth Yields and Maintenance Coefficients of Unadapted and NaCl-Adapted Tobacco Cells Grown in Semicontinuous Culture.
机构信息
Center for Plant Environmental Stress Physiology, Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
出版信息
Plant Physiol. 1991 Aug;96(4):1289-93. doi: 10.1104/pp.96.4.1289.
Comparison of carbon utilization between unadapted and NaCl (428 millimolar) adapted tobacco (Nicotiana tabacum L.) cells under substrate limited growth conditions was facilitated using semicontinuous culture. Growth yields (Y(g)) and maintenance coefficients (m) of unadapted and NaCl adapted cells were similar, indicating that the efficiency of carbon utilization for growth was not altered as a result of salt adaptation and that no additional metabolic costs were associated with growth of adapted cells in the presence of a high concentration (428 millimolar) of NaCl. The Y(g) (0.588 grams organic dry weight gain per gram sugar uptake) and m values (0.117 grams sugar uptake per gram organic dry weight per day) were comparable in spite of substantial physiological and biochemical differences that exist between unadapted and NaCl adapted cells. Apparently, a metabolic homeostasis governs biomass production of cells before and after adaptation to salinity.
在底物限制生长条件下，使用半连续培养法比较了未经驯化和经 428 毫摩尔 NaCl 驯化的烟草（Nicotiana tabacum L.）细胞之间的碳利用情况。未经驯化和经 NaCl 驯化的细胞的生长产率（Y(g)）和维持系数（m）相似，表明碳利用效率不因盐驯化而改变，并且在高浓度（428 毫摩尔）NaCl 存在下，适应细胞的生长没有与代谢相关的额外成本。尽管未经驯化和经 NaCl 驯化的细胞之间存在显著的生理和生化差异，但 Y(g)（每克糖摄取量获得 0.588 克有机干重）和 m 值（每天每克有机干重摄取 0.117 克糖）相当。显然，在适应盐度之前和之后，代谢平衡控制着细胞的生物量生产。
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