Godoy-Hernández Gregorio, Loyola-Vargas Victor M
Departamento de Biología Experimental, División de Biología Vegetal, Centro de Investigación Científiíca de Yucatán, Apartado Postal 87, Cordemex, 97310, Yucatán, México.
Plant Cell Rep. 1997 Feb;16(5):287-290. doi: 10.1007/BF01088282.
Addition of various concentrations (0.5-20 mM) of acetylsalicylic acid (ASA) to tumor lines ofCatharanthus roseus cultivatedin vitro and requiring corn starch as carbon source, produced remarkable effects on secondary metabolite production. An increase of 505% total alkaloids per culture (cells plus liquid medium), 1587% total phenolics (liquid medium), 612% total furanocoumarins (liquid medium) and 1476% total anthocyanins (liquid medium) was detected. 1 mM ASA in combination with other elicitors, such as homogenates ofAspergillus fumigatus or trans-cinnamic acid, did not further increase the metabolite content substantially. The results suggest that ASA could act as a new biotic elicitor of metabolite production inC. roseus cell suspension culture.
向体外培养且以玉米淀粉作为碳源的长春花肿瘤细胞系中添加不同浓度（0.5 - 20 mM）的乙酰水杨酸（ASA），对其次生代谢产物的产生产生了显著影响。检测到每培养物（细胞加液体培养基）中总生物碱增加了505%，总酚类物质（液体培养基）增加了1587%，总呋喃香豆素（液体培养基）增加了612%，总花青素（液体培养基）增加了1476%。1 mM ASA与其他诱导子（如烟曲霉匀浆或反式肉桂酸）联合使用时，并未进一步显著提高代谢产物含量。结果表明，ASA可能作为长春花细胞悬浮培养中代谢产物产生的一种新型生物诱导子。
