Borejsza-Wysocki Wlodzimierz, Borejsza-Wysocka Ewa, Hrazdina Geza
Institute of Food Science, Cornell University, 14456-0462, Geneva, NY, USA.
Plant Cell Rep. 1997 Feb;16(5):304-309. doi: 10.1007/BF01088286.
Cell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased synthesis of pisatin. Phenylalanine ammonia-lyase, chalcone synthase and isoflavone reductase activities were present in untreated cells. Upon treatment with an elicitor preparation the activities of the first two enzymes showed a rapid, transient increase up to 20 hours after treatment. Isoflavone reductase showed a major and minor peak at 16 and 36 h, respectively, after elicitor treatment. The time course of the enzyme activity and pisatin accumulation is consistent with an elicitor-mediated response.
从发芽的豌豆（Pisum sativum L.）种子建立了细胞悬浮培养物。这种积累豌豆素的细胞培养物主要由含有一些细胞聚集体的单个细胞组成。这些细胞对酵母葡聚糖制剂处理有反应，细胞和培养基中豌豆素都会短暂积累。向细胞培养物中添加豌豆素导致豌豆素合成增加。未处理的细胞中存在苯丙氨酸解氨酶、查尔酮合酶和异黄酮还原酶活性。用诱导子制剂处理后，前两种酶的活性在处理后20小时内迅速、短暂增加。诱导子处理后，异黄酮还原酶分别在16小时和36小时出现一个主峰和一个次峰。酶活性和豌豆素积累的时间进程与诱导子介导的反应一致。
