Godwin I D, Sangduen N, Kunanuvatchaidach R, Piperidis G, Adkins S W
Department of Agriculture, The University of Queensland, 4072, Brisbane, Queensland, Australia.
Department of Genetics, Kasetsart University, 10900, Bangkok, Thailand.
Plant Cell Rep. 1997 Feb;16(5):320-324. doi: 10.1007/BF01088289.
RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar,indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental bands, and the appearance of novel non-parental bands. Segregation data revealed both heterozygous and homozygous mutation events, with recessive mutations more prevalent than dominant. All somaclonal families differed significantly from the parental material, indicating that genomic alterations occurred in all families regardless of phenotype. None of the variant families could be regarded as isogenic lines of FR13A at the DNA level. However, some of the DNA level variation may be in highly repeated sequences with no phenotypic effects. The implications for somaclonal breeding and genetic engineering programs are discussed.
对八个已知具有特定性状差异的水稻体细胞克隆系家族以及四个表型正常的体细胞克隆系家族进行了随机扩增多态性DNA（RAPD）分析。亲本品种籼稻品种FR13A在45个RAPD位点中，除一个位点外，其余位点均表现为同质纯合。在体细胞克隆系家族的45条带中，有28条带存在多态性，包括亲本带的缺失以及新的非亲本带的出现。分离数据显示了杂合和纯合突变事件，隐性突变比显性突变更普遍。所有体细胞克隆系家族均与亲本材料存在显著差异，这表明无论表型如何，所有家族均发生了基因组改变。在DNA水平上，没有一个变异家族可被视为FR13A的同基因系。然而，一些DNA水平的变异可能存在于高度重复序列中，且无表型效应。文中还讨论了体细胞克隆育种和基因工程项目的意义。
