Brown P T, Lange F D, Kranz E, Lörz H
Institut für Allgemeine Botanik, Hamburg, FRG.
Mol Gen Genet. 1993 Mar;237(3):311-7. doi: 10.1007/BF00279433.
We investigated the use of the polymerase chain reaction (PCR) and the associated random amplification of polymorphic DNA (RAPD) technique in the analysis of DNA and specific genes in plant cells at different stages of regeneration in in vitro cultures. We demonstrate that both procedures can be used to differentiate reproducibly between closely related species as well as to reveal levels of DNA polymorphism in regenerated plants. We also demonstrate that both procedures, using protocols that we have developed, are applicable at all tissue culture stages, from single isolated protoplasts to regenerated plants. Possible explanations for the variation levels detected in regenerated wheat plants are advanced.
我们研究了聚合酶链反应（PCR）及相关的随机扩增多态性DNA（RAPD）技术在体外培养不同再生阶段植物细胞的DNA和特定基因分析中的应用。我们证明，这两种方法都可用于在亲缘关系相近的物种之间进行可重复的区分，以及揭示再生植株中的DNA多态性水平。我们还证明，使用我们开发的方案，这两种方法适用于从单个分离原生质体到再生植株的所有组织培养阶段。文中还提出了对再生小麦植株中检测到的变异水平的可能解释。
