Gupta Kuldeep, Singh Salam Pradeep, Manhar Ajay Kumar, Saikia Devabrata, Namsa Nima D, Konwar Bolin Kumar, Mandal Manabendra
Department of Molecular Biology and Biotechnology, Tezpur University (A Central University), Napaam, Tezpur, Assam 784028 India.
Indian J Microbiol. 2019 Mar;59(1):13-21. doi: 10.1007/s12088-018-0770-9. Epub 2018 Dec 6.
L. Skeels (Myretacae family) is a native plant of the Indian subcontinent which has wide socio-economical importance and is well known for its ant diabetic activity. The present study aimed to investigate the antibiofilm activity of purified fraction (EA) from leaf extract against and . The EA did not show any effect on growth of and at the concentration of 900 µg/ml. At this concentration EA showed biofilm inhibition up to 86 ± 1.19% (*** < 0.0001) and 86.40 ± 1.19% (*** < 0.0001) in and respectively. SEM examination also confirmed the reduction in biofilm formation. Further EA also disrupted some virulence phenotypes in and . Bioactive compounds detected by GC-MS showed their possible molecular interaction with RhlG/NADP active-site complex (PDB ID: 2B4Q), LasR-TP4 complex (PDB ID: 3JPU) and Pseudaminidase (PDB ID: 2W38) from The in vitro biofilm inhibition, virulence factor inhibition and the mode of interaction of bioactive components in with QS proteins of bacteria reported in this study might be an affordable and effective alternative method of controlling quorum sensing/biofilm-associated infections.
L. Skeels（Myretacae科）是印度次大陆的一种本土植物，具有广泛的社会经济重要性，并且以其抗糖尿病活性而闻名。本研究旨在调查叶提取物的纯化组分（EA）对[具体细菌名称1]和[具体细菌名称2]的抗生物膜活性。在900μg/ml的浓度下，EA对[具体细菌名称1]和[具体细菌名称2]的生长没有显示出任何影响。在此浓度下，EA在[具体细菌名称1]和[具体细菌名称2]中分别显示出高达86±1.19%（<0.0001）和86.40±1.19%（<0.0001）的生物膜抑制作用。扫描电子显微镜检查也证实了生物膜形成的减少。此外，EA还破坏了[具体细菌名称1]和[具体细菌名称2]中的一些毒力表型。气相色谱-质谱联用检测到的生物活性化合物显示它们可能与来自[具体细菌名称1]的RhlG/NADP活性位点复合物（PDB ID：2B4Q）、LasR-TP4复合物（PDB ID：3JPU）和假氨基肽酶（PDB ID：2W38）发生分子相互作用。本研究报道的[具体细菌名称1]中生物活性成分的体外生物膜抑制、毒力因子抑制以及与细菌群体感应蛋白的相互作用模式可能是控制群体感应/生物膜相关感染的一种经济有效的替代方法。
