Inhibition of and Biofilm and Virulence by Active Fraction of (L.) Skeels Leaf Extract: In-Vitro and In Silico Studies.

L. Skeels (Myretacae family) is a native plant of the Indian subcontinent which has wide socio-economical importance and is well known for its ant diabetic activity. The present study aimed to investigate the antibiofilm activity of purified fraction (EA) from leaf extract against and . The EA did not show any effect on growth of and at the concentration of 900 µg/ml. At this concentration EA showed biofilm inhibition up to 86 ± 1.19% (*** < 0.0001) and 86.40 ± 1.19% (*** < 0.0001) in and respectively. SEM examination also confirmed the reduction in biofilm formation. Further EA also disrupted some virulence phenotypes in and . Bioactive compounds detected by GC-MS showed their possible molecular interaction with RhlG/NADP active-site complex (PDB ID: 2B4Q), LasR-TP4 complex (PDB ID: 3JPU) and Pseudaminidase (PDB ID: 2W38) from The in vitro biofilm inhibition, virulence factor inhibition and the mode of interaction of bioactive components in with QS proteins of bacteria reported in this study might be an affordable and effective alternative method of controlling quorum sensing/biofilm-associated infections.