Rahman Md Ramim Tanver, Lou Zaixiang, Yu Fuhao, Wang Peng, Wang Hongxin
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Research Center of Food Nutrition & Functional Factors, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Research Center of Food Nutrition & Functional Factors, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Department of Food Science and Technology, University of California, Davis, USA.
Saudi J Biol Sci. 2017 Feb;24(2):324-330. doi: 10.1016/j.sjbs.2015.09.034. Epub 2015 Oct 9.
Cell-to-cell communication or quorum sensing (QS) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. This study evaluated the anti-QS activity of the extract (0.5-4 mg/ml) by using a biosensor strain and biofilm formation by crystal violate assay. Experimental results demonstrated that the overall yield of extract was 11.33 ± 0.3% (w/w). MIC for (Gram positive), Typhimurium (Gram negative) was 1, 2 and 2 mg/ml, respectively. A concentration of 4 mg/ml extract showed highest biofilm inhibition 51.96% on Typhimurium when 47.06%, 45.28% were shown by , respectively The damage of biofilm architecture was observed by Confocal Laser Scanning Microscopy (CLSM). A level of 44.59% inhibition of violacein production was demonstrated when the dose was 4 mg/ml. Swarming motility inhibition was observed in a dose dependent manner. Taken together, the treatment of extract can deliver value to food product and medicine by controlling pathogenesis.
细胞间通讯或群体感应（QS）会导致生物膜形成并引发其他毒力因子，这对食品安全、生物膜相关传染病等来说是极其严重的问题。本研究通过使用生物传感器菌株评估了提取物（0.5 - 4毫克/毫升）的抗群体感应活性，并通过结晶紫测定法评估了生物膜形成情况。实验结果表明，提取物的总产率为11.33 ± 0.3%（w/w）。提取物对金黄色葡萄球菌（革兰氏阳性菌）、鼠伤寒沙门氏菌（革兰氏阴性菌）的最低抑菌浓度分别为1、2和2毫克/毫升。当提取物浓度为4毫克/毫升时，对鼠伤寒沙门氏菌的生物膜抑制率最高，为51.96%，而对金黄色葡萄球菌和大肠杆菌的生物膜抑制率分别为47.06%和45.28%。通过共聚焦激光扫描显微镜（CLSM）观察到生物膜结构受到破坏。当剂量为4毫克/毫升时，紫菌素产量的抑制水平为44.59%。观察到提取物对群游运动的抑制呈剂量依赖性。综上所述，提取物处理可通过控制发病机制为食品和药品带来价值。
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