Molecular Genetics and Genomics Program, Division of Biology and Biomedical Sciences, Washington University, St. Louis, MO 63110, USA.
Department of Genetics, Washington University, St. Louis, MO 63110, USA.
Sci Adv. 2019 Jan 30;5(1):eaav1848. doi: 10.1126/sciadv.aav1848. eCollection 2019 Jan.
Genetic analysis of phenotypic differences between species is typically limited to interfertile species. Here, we conducted a genome-wide noncomplementation screen to identify genes that contribute to a major difference in thermal growth profile between two reproductively isolated yeast species, and . The screen identified only a single nuclear-encoded gene with a moderate effect on heat tolerance, but, in contrast, revealed a large effect of mitochondrial DNA (mitotype) on both heat and cold tolerance. Recombinant mitotypes indicate that multiple genes contribute to thermal divergence, and we show that protein divergence in affects both heat and cold tolerance. Our results point to the yeast mitochondrial genome as an evolutionary hotspot for thermal divergence.
种间表型差异的遗传分析通常仅限于可杂交物种。在这里，我们进行了全基因组非互补性筛选，以鉴定导致两种生殖隔离酵母物种和之间热生长曲线差异的主要基因。该筛选仅鉴定出一个对耐热性有中等影响的单个核编码基因，但相比之下，线粒体 DNA（线粒体型）对热和冷耐受性都有很大影响。重组线粒体型表明多个基因对热适应性有贡献，我们表明影响热和冷耐受性的酵母中的蛋白质差异。我们的结果表明，酵母线粒体基因组是热适应性进化热点。
