• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

H5N8的灭活H5抗原可保护鸡免受高致病性H5N8和H5N6禽流感病毒的致死性感染。

Inactivated H5 Antigens of H5N8 Protect Chickens from Lethal Infections by the Highly Pathogenic H5N8 and H5N6 Avian Influenza Viruses.

作者信息

Jin Myongha, Jang Yunyueng, Seo Taehyun, Seo Sang Heui

机构信息

Laboratory of Influenza Research, Umuahia Abia State, Republic of Korea.

Institute of Influenza Virus, College of Veterinary Medicine,Chungnam National University, Daejeon 34134, Republic of Korea, Umuahia Abia State, Republic of Korea.

出版信息

J Vet Res. 2018 Dec 31;62(4):413-420. doi: 10.2478/jvetres-2018-0078. eCollection 2018 Dec.

DOI:10.2478/jvetres-2018-0078
PMID:30729196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6364154/
Abstract

INTRODUCTION

Highly pathogenic Asian H5-subtype avian influenza viruses have been found in poultry and wild birds worldwide since they were first detected in southern China in 1996. Extensive control efforts have not eradicated them. Vaccination prevents such viruses infecting poultry and reduces the number lost to compulsory slaughter. The study showed the efficacy of inactivated H5 vaccine from the H5N8 virus against highly pathogenic H5N8 and H5N6 avian influenza viruses in chickens.

MATERIAL AND METHODS

Reverse genetics constructed an H5 vaccine virus using the HA gene of the 2014 H5N8 avian influenza virus and the rest of the genes from A/PR/8/34 (H1N1). The vaccine viruses were grown in fertilised eggs, partially purified through a sucrose gradient, and inactivated with formalin. Chickens were immunised i.m. with 1 μg of oil-adjuvanted inactivated H5 antigens.

RESULTS

Single dose H5 vaccine recipients were completely protected from lethal infections by homologous H5N8 avian influenza virus and shed no virus from the respiratory or intestinal tracts but were not protected from lethal infections by heterologous H5N6. When chickens were immunised with two doses and challenged with homologous H5N8 or heterologous H5N6, all survived and shed no virus.

CONCLUSION

Our results indicate that two-dose immunisations of chickens with H5 antigens with oil adjuvant are needed to provide broad protection against different highly pathogenic H5 avian influenza viruses.

摘要

引言

自1996年在中国南方首次发现高致病性亚洲H5亚型禽流感病毒以来，在全球范围内的家禽和野鸟中均有发现。广泛的防控措施未能根除这些病毒。疫苗接种可预防此类病毒感染家禽，并减少因强制扑杀而损失的数量。该研究显示了来自H5N8病毒的灭活H5疫苗对鸡群中高致病性H5N8和H5N6禽流感病毒的有效性。

材料与方法

利用2014年H5N8禽流感病毒的血凝素（HA）基因和A/PR/8/34（H1N1）的其余基因，通过反向遗传学构建了一种H5疫苗病毒。疫苗病毒在受精蛋中培养，经蔗糖梯度进行部分纯化，并用福尔马林灭活。给鸡肌肉注射1μg油佐剂灭活H5抗原进行免疫。

结果

单剂量接种H5疫苗的鸡完全受到同源H5N8禽流感病毒致死性感染的保护，呼吸道和肠道均未排毒，但未受到异源H5N6致死性感染的保护。当鸡接种两剂疫苗并用同源H5N8或异源H5N6攻击时，所有鸡均存活且未排毒。

结论

我们的结果表明，需要用H5抗原和油佐剂对鸡进行两剂免疫，以提供针对不同高致病性H5禽流感病毒的广泛保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/1210952e8e05/jvetres-62-413-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/9899d79563f3/jvetres-62-413-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/68169fa30ce8/jvetres-62-413-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/a5f67cfdb0f7/jvetres-62-413-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/35edebe40158/jvetres-62-413-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/1210952e8e05/jvetres-62-413-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/9899d79563f3/jvetres-62-413-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/68169fa30ce8/jvetres-62-413-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/a5f67cfdb0f7/jvetres-62-413-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/35edebe40158/jvetres-62-413-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2817/6364154/1210952e8e05/jvetres-62-413-g005.jpg

相似文献

1
Inactivated H5 Antigens of H5N8 Protect Chickens from Lethal Infections by the Highly Pathogenic H5N8 and H5N6 Avian Influenza Viruses.H5N8的灭活H5抗原可保护鸡免受高致病性H5N8和H5N6禽流感病毒的致死性感染。
J Vet Res. 2018 Dec 31;62(4):413-420. doi: 10.2478/jvetres-2018-0078. eCollection 2018 Dec.
2
Evaluation of Efficacy of Oil Adjuvanted H5N6 Inactivated Vaccine against Highly Pathogenic H5N6 and H5N1 Influenza Viruses Infected Chickens.油佐剂 H5N6 灭活疫苗对高致病性 H5N6 和 H5N1 流感病毒感染鸡的疗效评价。
Front Biosci (Landmark Ed). 2022 Sep 28;27(9):268. doi: 10.31083/j.fbl2709268.
3
Protective Efficacy of an H5N1 Inactivated Vaccine Against Challenge with Lethal H5N1, H5N2, H5N6, and H5N8 Influenza Viruses in Chickens.一种H5N1灭活疫苗对鸡抵抗致死性H5N1、H5N2、H5N6和H5N8流感病毒攻击的保护效力
Avian Dis. 2016 May;60(1 Suppl):253-5. doi: 10.1637/11179-052015-ResNoteR.
4
Vaccine Efficacy of Inactivated, Chimeric Hemagglutinin H9/H5N2 Avian Influenza Virus and Its Suitability for the Marker Vaccine Strategy.灭活嵌合血凝素H9/H5N2禽流感病毒的疫苗效力及其对标记疫苗策略的适用性
J Virol. 2017 Feb 28;91(6). doi: 10.1128/JVI.01693-16. Print 2017 Mar 15.
5
Homologous and heterologous antigenic matched vaccines containing different H5 hemagglutinins provide variable protection of chickens from the 2014 U.S. H5N8 and H5N2 clade 2.3.4.4 highly pathogenic avian influenza viruses.含有不同 H5 血凝素的同源和异源抗原匹配疫苗可提供不同程度的保护，使鸡免受 2014 年美国 H5N8 和 H5N2 2.3.4.4 高致病性禽流感病毒的侵害。
Vaccine. 2017 Nov 1;35(46):6345-6353. doi: 10.1016/j.vaccine.2017.04.042. Epub 2017 Apr 26.
6
Bivalent Hemagglutinin Cleavage-Site Peptide Vaccines Protect Chickens from Lethal Infections with Highly Pathogenic H5N1 and H5N6 Avian Influenza Viruses.二价血凝素裂解位点肽疫苗可预防高致病性 H5N1 和 H5N6 禽流感病毒对鸡的致死性感染。
Front Biosci (Landmark Ed). 2024 Feb 6;29(2):61. doi: 10.31083/j.fbl2902061.
7
Examination of the protective efficacy of two avian influenza H5 vaccines against clade 2.3.4.4b H5N8 highly pathogenic avian influenza virus in commercial broilers.检测两种禽流感 H5 疫苗对商业肉鸡中 2.3.4.4b 分支 H5N8 高致病性禽流感病毒的保护效力。
Res Vet Sci. 2021 Nov;140:125-133. doi: 10.1016/j.rvsc.2021.08.012. Epub 2021 Aug 17.
8
Newcastle disease virus-based H5 influenza vaccine protects chickens from lethal challenge with a highly pathogenic H5N2 avian influenza virus.基于新城疫病毒的H5流感疫苗可保护鸡免受高致病性H5N2禽流感病毒的致死性攻击。
NPJ Vaccines. 2017 Dec 4;2:33. doi: 10.1038/s41541-017-0034-4. eCollection 2017.
9
Comparison of the pathogenic potential of highly pathogenic avian influenza (HPAI) H5N6, and H5N8 viruses isolated in South Korea during the 2016-2017 winter season.比较 2016-2017 年冬季韩国分离的高致病性禽流感（HPAI）H5N6 和 H5N8 病毒的致病潜力。
Emerg Microbes Infect. 2018 Mar 14;7(1):29. doi: 10.1038/s41426-018-0029-x.
10
Prime-Boost Vaccination With a Novel Hemagglutinin Protein Produced in Bacteria Induces Neutralizing Antibody Responses Against H5-Subtype Influenza Viruses in Commercial Chickens.用细菌生产的新型血凝素蛋白进行 Prime-Boost 疫苗接种可诱导商业肉鸡产生针对 H5 亚型流感病毒的中和抗体反应。
Front Immunol. 2019 Sep 4;10:2006. doi: 10.3389/fimmu.2019.02006. eCollection 2019.

引用本文的文献

1
The potency of newly development H5N8 and H9N2 avian influenza vaccines against the isolated strains in laying hens from Egypt during 2019.2019年新研发的H5N8和H9N2禽流感疫苗对从埃及蛋鸡中分离出的毒株的效力。
Saudi J Biol Sci. 2021 Sep;28(9):5310-5316. doi: 10.1016/j.sjbs.2021.05.049. Epub 2021 May 24.

本文引用的文献

1
Influenza D Virus Infection in Feral Swine Populations, United States.美国野猪群体中感染 D 型流感病毒。
Emerg Infect Dis. 2018 Jun;24(6):1020-1028. doi: 10.3201/eid2406.172102.
2
Emergence and spread of highly pathogenic avian influenza A(H5N8) in Europe in 2016-2017.2016-2017 年在欧洲出现和传播的高致病性禽流感 A(H5N8)。
Transbound Emerg Dis. 2018 Oct;65(5):1217-1226. doi: 10.1111/tbed.12861. Epub 2018 Mar 14.
3
Airborne Detection of H5N8 Highly Pathogenic Avian Influenza Virus Genome in Poultry Farms, France.
法国家禽养殖场中H5N8高致病性禽流感病毒基因组的空气传播检测
Front Vet Sci. 2018 Feb 13;5:15. doi: 10.3389/fvets.2018.00015. eCollection 2018.
4
Multiple Introductions of Influenza A(H5N8) Virus into Poultry, Egypt, 2017.2017 年埃及多次发生 H5N8 型流感病毒向家禽的传播。
Emerg Infect Dis. 2018 May;24(5):943-6. doi: 10.3201/eid2405.171935. Epub 2018 May 17.
5
Novel Highly Pathogenic Avian Influenza A(H5N6) Virus in the Netherlands, December 2017.2017年12月在荷兰发现的新型高致病性甲型禽流感病毒（H5N6）
Emerg Infect Dis. 2018 Apr 17;24(4):770-3. doi: 10.3201/eid2404.172124.
6
Containment of Highly Pathogenic Avian Influenza A(H5N1) Virus, Lebanon, 20161.2016 年黎巴嫩高致病性禽流感病毒（H5N1）的控制
Emerg Infect Dis. 2018 Feb;24(2):374-376. doi: 10.3201/eid2402.171276.
7
Influenza D Virus in Cattle, Ireland.牛源 D 型流感病毒，爱尔兰。
Emerg Infect Dis. 2018 Feb;24(2):389-391. doi: 10.3201/eid2402.170759.
8
Newcastle disease virus-based H5 influenza vaccine protects chickens from lethal challenge with a highly pathogenic H5N2 avian influenza virus.基于新城疫病毒的H5流感疫苗可保护鸡免受高致病性H5N2禽流感病毒的致死性攻击。
NPJ Vaccines. 2017 Dec 4;2:33. doi: 10.1038/s41541-017-0034-4. eCollection 2017.
9
Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses.单剂量基于血凝素的共识病毒样颗粒疫苗可保护鸡免受不同H5亚型流感病毒的侵害。
Front Immunol. 2017 Nov 27;8:1649. doi: 10.3389/fimmu.2017.01649. eCollection 2017.
10
Multiple Reassorted Viruses as Cause of Highly Pathogenic Avian Influenza A(H5N8) Virus Epidemic, the Netherlands, 2016.2016 年，荷兰高致病性禽流感 A(H5N8)病毒疫情的多重重排病毒病因。
Emerg Infect Dis. 2017 Dec;23(12):1974-1981. doi: 10.3201/eid2312.171062.