Małaczewska Joanna, Kaczorek-Łukowska Edyta, Szymańska-Czerwińska Monika, Rękawek Wojciech, Wójcik Roman, Niemczuk Krzysztof, Siwicki Andrzej Krzysztof
Department of Microbiology and Clinical Immunology, cracow, Poland.
Department of Cattle and Sheep Diseases/Laboratory of Serological Diagnosis, National Veterinary Research Institute, 24-100, Pulawy, Poland.
J Vet Res. 2018 Dec 31;62(4):469-476. doi: 10.2478/jvetres-2018-0076. eCollection 2018 Dec.
(.) , the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines.
The purpose of this research was to determine the levels of the cytokines that play a key role in the response to antigens (IL-1β, IL-2, IL-6, IL-10, IFN-γ and TNF-α) in the serum of animals originating from an infected herd prior to vaccination (day 0) and at 1, 7, and 21 days afterwards.
The vaccination of animals did not affect the production of IL-6, IL-1β, or IL-2. The serum levels of these cytokines were too low to measure in most of the samples. The initial levels of TNFα, IFNγ, and IL-10 were higher in seropositive than in seronegative animals, although significant differences between seropositive shedders and seropositive nonshedders appeared only in the levels of IFNγ and IL-10. Additionally, the course of the post-vaccination response concerning these two cytokines was different among seronegative nonshedders, seropositive nonshedders, and seropositive shedders.
It seems that analysis of the IFNγ and IL-10 concentrations in animal blood serum may have some practical value in an assessment of the health status of seropositive animals and post-vaccination response.
Q热的病原体能够调节受感染机体中的巨噬细胞/T淋巴细胞轴，并损害单核因子和淋巴因子的合成。
本研究的目的是测定在接种疫苗前（第0天）以及接种后第1、7和21天，来自受感染畜群的动物血清中在对抗抗原反应中起关键作用的细胞因子（IL-1β、IL-2、IL-6、IL-10、IFN-γ和TNF-α）的水平。
动物接种疫苗并未影响IL-6、IL-1β或IL-2的产生。在大多数样本中，这些细胞因子的血清水平过低无法测量。血清阳性动物中TNFα、IFNγ和IL-10的初始水平高于血清阴性动物，尽管血清阳性排菌者和血清阳性非排菌者之间的显著差异仅出现在IFNγ和IL-10的水平上。此外，血清阴性非排菌者、血清阳性非排菌者和血清阳性排菌者中这两种细胞因子的接种后反应过程有所不同。
动物血清中IFNγ和IL-10浓度的分析似乎在评估血清阳性动物的健康状况和接种后反应方面具有一定的实用价值。
