Żarczyńska Katarzyna, Sobiech Przemysław, Snarska Anna, Tobolski Dawid, Shekhar Pareek Chandra, Bednarek Dariusz
Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
J Vet Res. 2018 Dec 31;62(4):503-509. doi: 10.2478/jvetres-2018-0072. eCollection 2018 Dec.
In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by determining the activity of creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The aim of this study was to analyse variations in the serum levels of LDH isoenzymes in calves with nutritional muscular dystrophy (NMD), to determine the applicability of this parameter for diagnosing NMD, and to describe the influence of hyposelenosis on total protein (TP), triglyceride (TG), and cholesterol (CHOL) levels.
Two groups of calves (n = six animals per group) were used. After birth, control group calves (SC) were intramuscularly administered 10 ml of a preparation containing selenium (Se) and vitamin E, and experimental group animals (SE) that were not injected. Blood was collected after 5, 15, and 25 days, and the concentrations of Se, vitamin E, TP, TG, and CHOL and the activity of glutathione peroxidase (GSH-Px), CK, and LDH fractions were determined.
Hypocholesterolaemia and elevated TG levels were found in SE group calves whose LDH fractions revealed a significant increase in LDH and LDH activity and a decrease in LDH activity when electrophoretically separated.
Nutritional muscular dystrophy is accompanied by hypocholesterolaemia and elevated TG levels caused by muscle lipolysis. LDH and LDH activity parameters assist early diagnosis of NMD in calves.
在犊牛中，低硒症会使身体不同部位的骨骼肌退化。通过测定肌酸激酶（CK）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）的活性，可以诊断肌肉细胞的损伤程度。本研究的目的是分析营养性肌营养不良（NMD）犊牛血清中LDH同工酶水平的变化，确定该参数在诊断NMD中的适用性，并描述低硒症对总蛋白（TP）、甘油三酯（TG）和胆固醇（CHOL）水平的影响。
使用两组犊牛（每组n = 6只动物）。出生后，对照组犊牛（SC）肌肉注射10 ml含硒（Se）和维生素E的制剂，实验组动物（SE）不注射。在第5、15和25天采集血液，测定Se、维生素E、TP、TG和CHOL的浓度以及谷胱甘肽过氧化物酶（GSH-Px）、CK和LDH组分的活性。
在SE组犊牛中发现了低胆固醇血症和TG水平升高，其LDH组分在电泳分离时显示LDH和LDH活性显著增加，而LDH活性降低。
营养性肌营养不良伴有因肌肉脂肪分解导致的低胆固醇血症和TG水平升高。LDH和LDH活性参数有助于早期诊断犊牛的NMD。
