Vasconcellos A F, Silva J M F, de Oliveira A S, Prado P S, Nagata T, Resende R O
Departamento de Biologia Celular, Bloco K Térreo, Instituto de Biologia, Universidade de Brasília Campus Darcy Ribeiro, Brasília, DF, CEP 70910-970, Brazil.
Laboratório Central de Saúde Pública do Distrito Federal (LACEN-DF), Setor de Grandes Áreas Norte, Quadras 601, Blocos Oe P., Brasília, DF, CEP 70830-010, Brazil.
Arch Virol. 2019 Apr;164(4):1205-1208. doi: 10.1007/s00705-019-04174-4. Epub 2019 Feb 7.
Chikungunya virus (CHIKV) is a reemerging arbovirus of the family Togaviridae that causes CHIKV fever, a disease that can extend from weeks to years depending on whether clinical signs of arthralgia persist. CHIKV is mainly transmitted by Aedes aegypti mosquitoes and possibly reached the Americas in 2013, causing an outbreak in Brazil in 2015. So far, two evolutionary lineages of CHIKV have been reported in Brazil: the Asian and the East-Central-South African (ECSA) lineages. In this study, six CHIKV isolates circulating in midwestern Brazil (Mato Grosso state) were isolated from patient sera, and their complete genomes were sequenced using a high-throughput sequencing platform. All of these isolates shared high nucleotide sequence similarity with CHIKV isolates from northeastern Brazil and were found to belong to the ECSA lineage. These CHIKV isolates did not contain the A226V or L210Q mutations that are associated with increased transmissibility by A. albopictus, suggesting that the CHIKV isolates circulating in midwestern Brazil are predominantly transmitted by A. aegypti.
基孔肯雅病毒（CHIKV）是披膜病毒科中一种再次出现的虫媒病毒，可引发基孔肯雅热，这种疾病的病程从数周持续到数年不等，具体取决于关节痛的临床症状是否持续存在。基孔肯雅病毒主要通过埃及伊蚊传播，2013年可能传入美洲，并于2015年在巴西引发疫情。目前，巴西已报告了基孔肯雅病毒的两个进化谱系：亚洲谱系和东中非-南非（ECSA）谱系。在本研究中，从巴西中西部（马托格罗索州）患者血清中分离出6株基孔肯雅病毒毒株，并使用高通量测序平台对其全基因组进行测序。所有这些毒株与巴西东北部的基孔肯雅病毒毒株具有高度的核苷酸序列相似性，且被发现属于ECSA谱系。这些基孔肯雅病毒毒株不包含与白纹伊蚊传播能力增强相关的A226V或L210Q突变，这表明在巴西中西部传播的基孔肯雅病毒毒株主要由埃及伊蚊传播。
