Kanczler Janos, Tare Rahul S, Stumpf Patrick, Noble Timothy J, Black Cameron, Oreffo Richard O C
Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Faculty of Medicine, Southampton, UK.
Methods Mol Biol. 2019;1914:53-70. doi: 10.1007/978-1-4939-8997-3_4.
In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1 and STRO-4 cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.
在本章中，我们描述了从人骨髓中分离和鉴定骨骼干细胞的技术。介绍了使用磁性激活细胞分选法富集STRO-1和STRO-4细胞的方法，并且我们还详细阐述了从这些细胞建立和鉴定成骨、成脂及成软骨培养物的技术。最后，我们展示了利用皮下植入小鼠体内的扩散小室研究这些细胞在体内生成骨的能力的方法。
