Gardner Oliver F W, Alini Mauro, Stoddart Martin J
Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.
Cardiff University School of Biosciences, Museum Avenue, Cardiff, UK.
Methods Mol Biol. 2015;1340:41-52. doi: 10.1007/978-1-4939-2938-2_3.
Mesenchymal stem cells are found in a number of tissues and have the potential to differentiate into a range of mesenchymal lineages. This ready availability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage. This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency.
间充质干细胞存在于多种组织中，具有分化为一系列间充质谱系的潜力。这种易于获取和多能性的特点意味着间充质干细胞已成为组织工程领域的焦点，特别是在骨和软骨修复方面。本章描述了从人骨髓组织中分离间充质干细胞，以及细胞的扩增和多能性的鉴定。
