Marino Silvia, Bishop Ryan T, de Ridder Daniëlle, Delgado-Calle Jesus, Reagan Michaela R
Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK.
Methods Mol Biol. 2019;1914:71-98. doi: 10.1007/978-1-4939-8997-3_5.
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.
共培养分析用于体外研究细胞间的相互作用。本章描述了用于研究癌细胞与骨髓脂肪细胞、成骨细胞、破骨细胞和骨细胞之间细胞间信号串扰的二维和三维共培养系统。本章提供了最常用的细胞培养技术、功能分析和基因表达的分步指南。
