用于癌症与骨细胞相互作用研究的二维和三维体外共培养
2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies.
作者信息
Marino Silvia, Bishop Ryan T, de Ridder Daniëlle, Delgado-Calle Jesus, Reagan Michaela R
机构信息
Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK.
出版信息
Methods Mol Biol. 2019;1914:71-98. doi: 10.1007/978-1-4939-8997-3_5.
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.
共培养分析用于体外研究细胞间的相互作用。本章描述了用于研究癌细胞与骨髓脂肪细胞、成骨细胞、破骨细胞和骨细胞之间细胞间信号串扰的二维和三维共培养系统。本章提供了最常用的细胞培养技术、功能分析和基因表达的分步指南。
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