Uluçkan Özge
Novartis Institutes for Biomedical Research, Basel, Switzerland.
Methods Mol Biol. 2019;1914:343-348. doi: 10.1007/978-1-4939-8997-3_19.
Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.
黑色素瘤是具有高转移能力的侵袭性皮肤癌。小鼠模型对于阐明癌症转移机制是必要的，而异种移植模型还可以利用不同的基因改造小鼠模型来研究宿主的作用。在本章中，我提供了详细的方案，用于通过心脏内和胫骨内接种肿瘤细胞以实现骨转移。
