Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, INICSA, De La Reforma Bv., Ciudad Universitaria, Córdoba, Argentina.
Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, De La Reforma Bv., Ciudad Universitaria, Córdoba, Argentina.
Phytother Res. 2019 Apr;33(4):1142-1149. doi: 10.1002/ptr.6308. Epub 2019 Feb 6.
Lung carcinoma is one of the most common cancers and has a high mortality. Recently, we showed that it produces neurological paraneoplastic syndrome, with Ilex paraguariensis (IP) extract exerting palliative effects due to its content of phenolic compounds. It is possible, therefore, that these diet agents can arrive at the brain and exert neuroprotection, after the oral intake of IP. Here, the aim was to investigate the protective role of bioavailable IP compounds on the telencephalon and diencephalon in lung adenocarcinoma-bearing BALB/cJ males. Mice aged 2 months were treated for 3 weeks with 0-100 IP mg·kg ·day . HPLC-UV revealed the presence of chlorogenic acid and quercetin in brain regions, liver, and tumour, in an IP dose-dependent manner. Brain was also evaluated histologically, and interleukin-6 was measured by ELISA. Chlorogenic acid was the major compound found in brain, whereas quercetin was observed at the diencephalon to a lesser extent. Both compounds were involved in IP dose-dependent diencephalic interleukin-6 reduction. Histology suggested cellular protection with less apoptosis in chlorogenic-exposed areas. Taken together, chlorogenic acid and quercetin from dietary IP were bioavailable and bioactive in brain, thereby attenuating lung cancer-related neuroinflammation and damage. These findings support plant-based strategies to improve prognosis.
肺癌是最常见的癌症之一，死亡率很高。最近，我们发现它会产生神经副肿瘤综合征，巴拉圭冬青（IP）提取物因其含有酚类化合物而具有缓解作用。因此，这些饮食因子可能通过口服进入大脑并发挥神经保护作用。在这里，目的是研究生物可利用的 IP 化合物对携带肺癌的 BALB/cJ 雄性大脑皮质和间脑的保护作用。2 个月大的小鼠用 0-100 IP mg·kg·day 治疗 3 周。HPLC-UV 以 IP 剂量依赖性的方式显示出脑区、肝脏和肿瘤中存在绿原酸和槲皮素。还对大脑进行了组织学评估，并通过 ELISA 测量白细胞介素-6。绿原酸是大脑中发现的主要化合物，而槲皮素在间脑的含量较少。这两种化合物都参与了 IP 剂量依赖性的间脑白细胞介素-6 减少。组织学表明，在绿原酸暴露区域有细胞保护作用，凋亡减少。综上所述，饮食中的绿原酸和槲皮素从 IP 中是生物可利用和生物活性的，从而减轻与肺癌相关的神经炎症和损伤。这些发现支持基于植物的策略来改善预后。
