原发性抗磷脂抗体综合征患者的细胞凋亡

Apoptosis in patients with primary antiphospholipid antibody syndrome.

作者信息

Tang Kuo-Tung, Hsieh Tsu-Yi, Chao Ya-Hsuan, Li Ju-Pi, Lan Joung-Liang, Lin Chi-Chien, Chen Der-Yuan

机构信息

Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung, Taiwan.

Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, Taichung, Taiwan.

出版信息

Int J Rheum Dis. 2019 Apr;22(4):677-685. doi: 10.1111/1756-185X.13468. Epub 2019 Feb 6.

DOI:10.1111/1756-185X.13468

PMID:30729698

Abstract

AIM

Dysregulated apoptosis has been implicated in autoimmune diseases. In the present study, we investigated the apoptosis-related cytokines and apoptosis in patients with primary antiphospholipid syndrome (pAPS).

METHOD

We prospectively recruited 12 pAPS patients, 17 antiphospholipid antibody (APA)-positive systemic lupus erythematosus (SLE) patients without APS manifestations (APA SLE), 13 SLE patients with secondary APS (APS SLE) and 10 healthy controls (HCs). Plasma levels of soluble apoptosis-inducing ligands and cytokines, and the expression levels of apoptosis-inducing ligands in peripheral blood mononuclear cells, were determined. In addition, blood lymphocytes/monocytes apoptosis were determined in six pAPS patients and six HCs, using flow cytometric analysis of caspase 3, 8 and 9 activities.

RESULTS

There was a trend toward higher plasma levels of soluble tumor necrosis factor (TNF)-related apoptosis-inducing ligand (sTRAIL), interleukin-10 (IL-10) and TNF-α in pAPS patients when compared with HCs. We also observed higher plasma levels of IL-10 and TNF α in APA SLE and APS SLE patients when compared with HCs. However, there was no significant difference in blood lymphocytes/monocytes apoptosis between pAPS patients and HCs.

CONCLUSION

There was a trend toward elevated plasma levels of sTRAIL, IL-10 and TNF-α, but no evidence for dysregulated apoptosis in pAPS patients.

摘要

目的

细胞凋亡失调与自身免疫性疾病有关。在本研究中，我们调查了原发性抗磷脂综合征（pAPS）患者中与细胞凋亡相关的细胞因子及细胞凋亡情况。

方法

我们前瞻性招募了12例pAPS患者、17例无APS表现的抗磷脂抗体（APA）阳性系统性红斑狼疮（SLE）患者（APA-SLE）、13例继发性APS的SLE患者（APS-SLE）和10名健康对照者（HCs）。测定了血浆中可溶性凋亡诱导配体和细胞因子的水平，以及外周血单个核细胞中凋亡诱导配体的表达水平。此外，使用流式细胞术分析半胱天冬酶3、8和9的活性，测定了6例pAPS患者和6例HCs的血液淋巴细胞/单核细胞凋亡情况。

结果

与HCs相比，pAPS患者血浆中可溶性肿瘤坏死因子（TNF）相关凋亡诱导配体（sTRAIL）、白细胞介素-10（IL-10）和TNF-α水平有升高趋势。与HCs相比，我们还观察到APA-SLE和APS-SLE患者血浆中IL-10和TNF-α水平更高。然而，pAPS患者与HCs之间血液淋巴细胞/单核细胞凋亡无显著差异。