Ko Jung-Hwan, Rodriguez Isabel, Joo Sun-Woo, Kim Hyoung-Geun, Lee Yeong-Geun, Kang Tong Ho, Baek Nam-In
Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.
J Med Food. 2019 Feb;22(2):196-201. doi: 10.1089/jmf.2018.4235.
Malva verticillata (Chinese mallow) has long been used in traditional medicines and herbal teas in Asia. The n-BuOH fraction (Fr) from M. verticillata promoted significant recovery of alloxan-damaged (AXD) pancreatic islets (PIs) in zebrafish (ZF). Two major components were isolated from M. verticillata through repeated-column chromatography. Based on several spectroscopic methods, including nuclear magnetic resonance (NMR), infrared spectroscopy (IR), and fast atom bombardment-mass spectrometry (FAB-MS), the chemical structures of compounds 1 and 2 were determined. In addition, the quantity of both compounds in the n-BuOH Fr was investigated through high-performance liquid chromatography (HPLC) and the quantities of compounds 1 and 2 in the n-BuOH Fr were determined to be 5.58% ± 0.16% and 2.85% ± 0.13%, respectively. The n-BuOH Fr, compounds 1 and 2, and the mixture of compounds 1 and 2 (MX, 1 and 2, the ratio of both compounds in n-BuOH Fr, 1.96:1) were evaluated for their ability to recover AXD PIs and for their K channel-blocking mechanism using diazoxide in ZF. The n-BuOH Fr (10 μg/mL) and compounds 1 and MX (1 μg/mL) exhibited a recovery effect on AXD PIs. The n-BuOH Fr (10 μg/mL) and MX (1 μg/mL) were also confirmed to be useful K channel activators. A synergistic effect of MX in the recovery of AXD PIs was first confirmed in ZF, and it was discovered that 2 acted as an insulin sensitivity activator that increased the activity of compound 1.
冬葵在亚洲长期被用于传统药物和草药茶中。冬葵的正丁醇馏分促进了斑马鱼中被四氧嘧啶损伤的胰岛的显著恢复。通过反复柱色谱从冬葵中分离出两个主要成分。基于包括核磁共振（NMR）、红外光谱（IR）和快原子轰击质谱（FAB-MS）在内的几种光谱方法，确定了化合物1和2的化学结构。此外，通过高效液相色谱（HPLC）研究了正丁醇馏分中这两种化合物的含量，确定正丁醇馏分中化合物1和2的含量分别为5.58%±0.16%和2.85%±0.13%。使用二氮嗪在斑马鱼中评估了正丁醇馏分、化合物1和2以及化合物1和2的混合物（MX，1和2，正丁醇馏分中两种化合物的比例为1.96:1）恢复被四氧嘧啶损伤的胰岛的能力及其钾通道阻断机制。正丁醇馏分（10μg/mL）以及化合物1和MX（1μg/mL）对被四氧嘧啶损伤的胰岛表现出恢复作用。正丁醇馏分（10μg/mL）和MX（1μg/mL）也被证实是有用的钾通道激活剂。首次在斑马鱼中证实了MX在恢复被四氧嘧啶损伤的胰岛方面具有协同作用，并且发现化合物2作为胰岛素敏感性激活剂增加了化合物1的活性。
