Huhtanen C N, Phillips J G, Brazis A R, Bredvold E, Marshall R T, Pederson D E, Sing E L, Thompson D I, Waltz M F, Wehr H M, Bengsch H
J Food Prot. 1977 Apr;40(4):236-240. doi: 10.4315/0362-028X-40.4.236.
Eleven analysts tested contaminated reconstituted (1:10) dry milk powders for penicillin residues using spores of Bacillus stearothermophilus var. calidolacris (Delvotest P method). Three types of responses were noted: positive, negative, and questionable. Prediction equations indicated that 95% of the time, analysts unfamiliar with the technique could detect positive results if penicillin concentrations in samples were 0.010 unit/ml or higher and positive and questionable results if the penicillin concentrations were 0.008 unit/ml. Increasing the reconstitution ratio from 1/11 to 1/4 increased the chances of detecting penicillin in milk powder. Penicillinase added to reconstituted penicillin-contaminated milks in all instances produced negative responses.
11名分析人员使用嗜热脂肪芽孢杆菌变种嗜热栖热菌（Delvotest P法）的孢子检测了受污染的复溶（1:10）奶粉中的青霉素残留。记录了三种类型的反应：阳性、阴性和可疑。预测方程表明，在95%的情况下，如果样品中的青霉素浓度为0.010单位/毫升或更高，不熟悉该技术的分析人员可以检测到阳性结果；如果青霉素浓度为0.008单位/毫升，则可以检测到阳性和可疑结果。将复溶比例从1/11提高到1/4增加了在奶粉中检测到青霉素的机会。在所有情况下，向复溶的受青霉素污染的牛奶中添加青霉素酶都会产生阴性反应。
