Senyk Gary F, Davidson Joseph H, Brown Janice M, Hallstead Eric R, Sherbon John W
Department of Food Science, Cornell University, Ithaca, New York 14853.
J Food Prot. 1990 Feb;53(2):158-164. doi: 10.4315/0362-028X-53.2.158.
Five rapid methods for detection of antibiotics in milk were compared. The Bacillus stearothermophilus var. calidolactis disc assay was also performed on the same samples. The rapid methods were: Angenics Spot Test, Charm II, Delvotest P, Penzyme Farm, and Penzyme Lab III. Ten antibiotics (penicillin G, cephapirin, cloxacillin, ampicillin, streptomycin, chloramphenicol, erythromycin, novobiocin, tetracycline, and gentamicin) were used individually to spike eight raw milk samples at five levels of antibiotic. Antibiotic levels were chosen that would result in zones of <16 mm, 16 mm, and >16 mm on the disc assay. Only the disc assay, Charm II and Delvotest P were compared on non-beta-lactam antibiotics. A small percentage of milks with no antibiotic added tested positive with the Charm II and Penzyme Lab III. On combined data for penicillin G, cephapirin, and cloxacillin, for which all methods were compared, the percent correctly categorized as pass (below actionable) for the <16 mm zone spiked level, reject or caution at the 16 mm zone level, and reject or caution at the >16 mm zone level were: Angenics 79, 83, 100; Charm II 66, 92, 100; Delvotest P 74, 93, 100; Disc Assay 100, 74, 100; Penzyme Farm 93, 61, 92; Penzyme Lab III 81,78, 100 respectively. In most cases, the rapid methods showed greater apparent sensitivity than the disc assay and did not fail to reject milks spiked with antibiotic in excess of the 16 mm zone level.
比较了五种快速检测牛奶中抗生素的方法。同时对相同的样品进行嗜热脂肪芽孢杆菌嗜热亚种圆盘检测。快速检测方法有：安杰尼克斯斑点试验、查姆II、德尔沃检测P、酶标农场法和酶标实验室III法。使用十种抗生素（青霉素G、头孢匹林、氯唑西林、氨苄青霉素、链霉素、氯霉素、红霉素、新生霉素、四环素和庆大霉素）分别以五种抗生素水平添加到八个生鲜乳样品中。选择的抗生素水平在圆盘检测中会产生小于16毫米、16毫米和大于16毫米的抑菌圈。仅对非β-内酰胺类抗生素比较了圆盘检测、查姆II和德尔沃检测P。一小部分未添加抗生素的牛奶在查姆II和酶标实验室III法检测中呈阳性。对于青霉素G、头孢匹林和氯唑西林的综合数据，所有方法都进行了比较，在抑菌圈小于16毫米的添加水平下正确分类为合格（低于可采取行动水平）、在16毫米抑菌圈水平下为拒收或警示、在大于16毫米抑菌圈水平下为拒收或警示的百分比分别为：安杰尼克斯79%、83%、100%；查姆II 66%、92%、100%；德尔沃检测P 74%、93%、100%；圆盘检测100%、74%、100%；酶标农场法93%、61%、92%；酶标实验室III法分别为81%、78%、100%。在大多数情况下，快速检测方法比圆盘检测表现出更高的表观灵敏度，并且不会漏检添加了超过16毫米抑菌圈水平抗生素的牛奶。
