Zhu Bin, Li Nanfang, Zhu Qing, Wu Ting, Heizati Mulalibieke, Wang Guoliang, Yao Xiaoguang, Luo Qin, Liu Shasha, Liu Shanshan, Hong Jing
Xinjiang Medical University.
Center for Hypertension of People's Hospital of Xinjiang Uygur Autonomous Region, Hypertension Institute of Xinjiang, Urumqi, Xinjiang, China.
Medicine (Baltimore). 2019 Feb;98(6):e14493. doi: 10.1097/MD.0000000000014493.
High mobility group box 1 (HMGB1) is a kind of proinflammatory mediator that acts as an alarmin when released by dying, injured or activated cells. Previous studies have reported that HMGB1 are closely linked to antineutrophil cytoplasmic antibody-associated vasculitis (AAV). The present study aimed to evaluate whether serum HMGB1 levels were associated with systemic vasculitis (VAs).The study population consisted of 51 patients with VAs, 46 patients with essential hypertension (EH) and 46 healthy controls (HC). Thirty-five patients with VAs had in active stage and 16 patients with VAs in an inactive stage. Furthermore, 31 patients with VAs had renal involvement, the other 20 patients were selected for without renal involvement. Serum HMGB1 levels were measured by enzyme-linked immunosorbent assay. Associations between serum HMGB1 levels with clinical and laboratory parameters were analyzed.Serum HMGB1 levels in patients with VAs were significantly higher than in EH and HC (all P < .05), and no difference regarding serum HMGB1 levels could be found between EH and HC (P = .208). Serum HMGB1 levels in VAs patients with active stage were significantly higher than those in HC and VAs patients with inactive stage (all P < .05). Patients with renal involvement and non-renal involvement had increased HMGB1 levels compared with HC (all P < .05). In addition, serum HMGB1 levels were significantly higher in patients with renal involvement compared with non-renal involvement patients (P = .001). Correlation analysis showed that serum HMGB1 levels were positive significant correlated with the Birmingham Vasculitis Activity Score, hypersensitive C reactive protein (Hs-CRP), serum creatinine (Scr) and 24-hour proteinuria (all P < .05). Among the subsets of VAs, serum HMGB1 levels were significantly higher in AAV, polyarteritis nodosa (PAN) and takayasu arteritis (TA) than in HC (all P < .05). More interestingly, serum HMGB1 were significantly higher in patients with PAN compared with AAV and TA patients (all P < .05). Furthermore, there was positive correlation between serum HMGB1 levels and Hs-CRP, Scr, and 24-hour proteinuria in patients with PAN (all P < .05).Serum HMGB1 levels are increased in patients with VAs compared with HC and EH and can reflect the disease activity and renal involvement.
高迁移率族蛋白B1（HMGB1）是一种促炎介质，当由死亡、受损或活化细胞释放时，它作为一种警报素发挥作用。先前的研究报道，HMGB1与抗中性粒细胞胞浆抗体相关性血管炎（AAV）密切相关。本研究旨在评估血清HMGB1水平是否与系统性血管炎（VAs）相关。研究人群包括51例VAs患者、46例原发性高血压（EH）患者和46例健康对照（HC）。35例VAs患者处于活动期，16例VAs患者处于非活动期。此外，31例VAs患者有肾脏受累，另外20例患者被选为无肾脏受累。采用酶联免疫吸附测定法测量血清HMGB1水平。分析血清HMGB1水平与临床和实验室参数之间的关联。VAs患者的血清HMGB1水平显著高于EH患者和HC（所有P<0.05），而EH患者和HC之间的血清HMGB1水平无差异（P=0.208）。活动期VAs患者的血清HMGB1水平显著高于HC和非活动期VAs患者（所有P<0.05）。与HC相比，有肾脏受累和无肾脏受累的患者HMGB1水平均升高（所有P<0.05）。此外，有肾脏受累的患者血清HMGB1水平显著高于无肾脏受累的患者（P=0.001）。相关性分析表明，血清HMGB1水平与伯明翰血管炎活动评分、超敏C反应蛋白（Hs-CRP）、血清肌酐（Scr）和24小时蛋白尿均呈显著正相关（所有P<0.05）。在VAs的亚组中，AAV、结节性多动脉炎（PAN）和大动脉炎（TA）患者的血清HMGB1水平显著高于HC（所有P<0.05）。更有趣的是，PAN患者的血清HMGB1水平显著高于AAV和TA患者（所有P<0.05）。此外，PAN患者的血清HMGB1水平与Hs-CRP、Scr和24小时蛋白尿之间存在正相关（所有P<0.05）。与HC和EH相比，VAs患者的血清HMGB1水平升高，且可反映疾病活动度和肾脏受累情况。
