Ruel David M, Yakir Esther, Bohbot Jonathan D
Department of Entomology, The Hebrew University of Jerusalem, Rehovot, Israel.
Front Cell Neurosci. 2019 Jan 24;12:533. doi: 10.3389/fncel.2018.00533. eCollection 2018.
Mosquitoes exhibit highly diverse and fast evolving odorant receptors (ORs). The indole-sensitive OR gene clade, comprised of and is a notable exception on account of its conservation in both mosquito subfamilies. This group of paralogous genes exhibits a complex developmental expression pattern in : is expressed in both adults and larvae, is adult-specific and a third member named is larva-specific. OR2 and OR10 have been deorphanized and are selectively activated by indole and skatole, respectively. Using the two-electrode voltage clamp of oocytes expressing . ORs, we show that AaegOR9 is supersensitive and narrowly tuned to skatole. Our findings suggest that . has evolved two distinct molecular strategies to detect skatole in aquatic and terrestrial environments, highlighting the central ecological roles of indolic compounds in the evolutionary and life histories of these insects.
蚊子表现出高度多样化且快速进化的气味受体（ORs）。由[具体基因名称1]和[具体基因名称2]组成的对吲哚敏感的OR基因进化枝是一个显著的例外，因为它在两个蚊子亚科中都具有保守性。这一组旁系同源基因在[具体物种名称]中表现出复杂的发育表达模式：[基因名称1]在成虫和幼虫中均有表达，[基因名称2]是成虫特异性的，而第三个名为[基因名称3]的成员是幼虫特异性的。OR2和OR10已被鉴定出功能，分别被吲哚和粪臭素选择性激活。利用表达[具体物种名称]ORs的卵母细胞的双电极电压钳技术，我们发现AaegOR9对粪臭素超敏感且具有狭窄的调谐范围。我们的研究结果表明，[具体物种名称]已经进化出两种不同的分子策略来检测水生和陆地环境中的粪臭素，突出了吲哚类化合物在这些昆虫的进化和生活史中的核心生态作用。
