中国中华按蚊的一种气味受体对产卵引诱剂敏感。
An odorant receptor from Anopheles sinensis in China is sensitive to oviposition attractants.
机构信息
Department of Medical Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, 272033, Shandong, People's Republic of China.
Jining Center for Disease Control and Prevention, Jining, 272033, Shandong, People's Republic of China.
出版信息
Malar J. 2018 Oct 5;17(1):348. doi: 10.1186/s12936-018-2501-4.
BACKGROUND
Anopheles sinensis is an important vector for the spread of malaria in China. Olfactory-related behaviours, particularly oviposition site seeking, offer opportunities for disrupting the disease-transmission process.
RESULTS
This is the first report of the identification and characterization of AsinOrco and AsinOR10 in An. sinensis. AsinOrco and AsinOR10 share 97.49% and 90.37% amino acid sequence identity, respectively, with related sequences in Anopheles gambiae. A functional analysis demonstrated that AsinOrco- and AsinOR10-coexpressing HEK293 cells were highly sensitive to 3-methylindole, but showed no significant differences in response to other test odorants when compared to DMSO.
CONCLUSIONS
AsinOrco was characterized as a new member of the Orco ortholog subfamily. AsinOR10, which appears to be a member of the OR2-10 subfamily, is directly involved in identification of oviposition sites. This finding will help to elucidate the molecular mechanisms underlying olfactory signaling in An. sinensis and provide many more molecular targets for eco-friendly pest control.
背景
中华按蚊是中国疟疾传播的重要媒介。嗅觉相关行为，特别是产卵场所寻找，为破坏疾病传播过程提供了机会。
结果
这是首次鉴定和描述中华按蚊 AsinOrco 和 AsinOR10 的报告。AsinOrco 和 AsinOR10 分别与疟蚊中的相关序列具有 97.49%和 90.37%的氨基酸序列同一性。功能分析表明，共表达 AsinOrco 和 AsinOR10 的 HEK293 细胞对 3-甲基吲哚高度敏感，但与 DMSO 相比，对其他测试气味剂没有明显的反应差异。
结论
AsinOrco 被鉴定为 Orco 直系同源亚科的新成员。AsinOR10 似乎是 OR2-10 亚科的成员，直接参与产卵场所的识别。这一发现将有助于阐明中华按蚊嗅觉信号转导的分子机制，并为环保型害虫控制提供更多的分子靶标。
Zotero 插件