三种酶水解蛋白的抗氧化和抗病毒活性
Antioxidant and antiviral activity of proteins hydrolyzed by three enzymes.
作者信息
Afify Abd El-Moneim M R, El Baroty Gamal S, El Baz Farouk K, Abd El Baky Hanaa H, Murad Soha A
机构信息
Biochemistry Department, Faculty of Agriculture, Cairo University, Cairo, Egypt.
Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
出版信息
J Genet Eng Biotechnol. 2018 Dec;16(2):399-408. doi: 10.1016/j.jgeb.2018.01.002. Epub 2018 Feb 1.
PURPOSE
To obtain protein hydrolysates from fresh water green algae by three different enzymes and evaluate its antioxidant and antiviral activity.
METHODS
Enzymatic hydrolysates of green algae protein were prepared by treatment with: 1.2% solution of pepsin, trypsin or papain. Protein was extracted from by three different extraction methods. Protein extracts and hydrolysates were assessed from stained gels following SDS-PAGE of samples. Antioxidant activity of protein hydrolysates was investigated.
RESULTS
cells and protein extracts were rich in Arg, Lys, Asp, Ala, and His. Protein hydrolyzed by papain (Sd1pa) and protein hydrolyzed by trypsin (Sd2Try) induced highest antioxidant activity based on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging (41.41% and 40.62%) respectively, and on 2,2'-azinobis 3-ethyl-benzothiazoline-6-sulphonate (ABTS) radical (87.03% and 45.12%) respectively, at 150 µg/ml. The inhibitory effect and mode of action of protein hydrolysates were evaluated against Coxsackie B virus (CVB). Protein hydrolyzed by papain (Sd2pa) and protein hydrolyzed by pepsin (Sd1pep) at 100 µg/ml exhibited antiviral activity (66.2% and 57.6%, respectively), against (CVB) from all protein hydrolysates.
CONCLUSION
protein hydrolysates have a potential as antioxidative neutraceutical ingredients and a potential therapeutic agent against CVB.
目的
通过三种不同的酶从淡水绿藻中获得蛋白质水解物，并评估其抗氧化和抗病毒活性。
方法
用1.2%的胃蛋白酶、胰蛋白酶或木瓜蛋白酶溶液处理制备绿藻蛋白的酶解物。通过三种不同的提取方法从绿藻中提取蛋白质。对样品进行SDS-PAGE后，从染色凝胶中评估蛋白质提取物和水解物。研究了蛋白质水解物的抗氧化活性。
结果
绿藻细胞和蛋白质提取物富含精氨酸、赖氨酸、天冬氨酸、丙氨酸和组氨酸。基于1,1-二苯基-2-苦基肼（DPPH）自由基清除能力，木瓜蛋白酶水解的蛋白质（Sd1pa）和胰蛋白酶水解的蛋白质（Sd2Try）分别在150µg/ml时诱导出最高的抗氧化活性（分别为41.41%和40.62%）；基于2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力，分别为87.03%和45.12%。评估了蛋白质水解物对柯萨奇B病毒（CVB）的抑制作用和作用方式。在100µg/ml时，木瓜蛋白酶水解的蛋白质（Sd2pa）和胃蛋白酶水解的蛋白质（Sd1pep）对所有蛋白质水解物中的CVB均表现出抗病毒活性（分别为66.2%和57.6%）。
结论
绿藻蛋白质水解物有作为抗氧化营养成分的潜力，以及作为抗CVB的潜在治疗剂的潜力。
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