Fouad Ali M, Gabr Mahmoud M, Abdelhady Elsayed K, Zakaria Mahmoud M, Khater Sherry M, Ismail Amani M, Refaie Ayman F
Department of Biotechnology, Urology and Nephrology Center, Mansoura 35516, Egypt.
Department of Zoology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
J Genet Eng Biotechnol. 2018 Dec;16(2):433-440. doi: 10.1016/j.jgeb.2018.09.003. Epub 2018 Dec 5.
Mesenchymal stem cells (MSCs) is a heterogeneous population. Muse cells is a rare pluripotent subpopulation within MSCs. This study aims to evaluate the pulirpotency and the ability of Muse cells to generate insulin producing cells (IPCs) after differentiation protocol compared to the non-Muse cells. Muse cells were isolated by FACSAria III cell sorter from adipose-derived MSCs and were evaluated for its pluripotency. Following differentiation, IPCs derived from Muse and non-Muse cells were evaluated for insulin production. Muse cells comprised 3.2 ± 0.7% of MSCs, approximately 82% of Muse cells were positive for anti stage-specific embryonic antigen-3 (SSEA-3). Pluripotent markers were highly expressed in Muse versus non-Muse cells. The percentage of generated IPCs by flow cytometric analysis was higher in Muse cells. Under confocal microscopy, Muse cells expressed insulin and c-peptide while it was undetected in non-Muse cells. Our results introduced Muse cells as a new adult pluripotent subpopulation, which is capable to produce higher number of functional IPCs.
间充质干细胞（MSCs）是一个异质性群体。多能分化应激耐受细胞（Muse细胞）是MSCs中一种罕见的多能亚群。本研究旨在评估Muse细胞与非Muse细胞相比，在分化方案后产生胰岛素生成细胞（IPCs）的多能性和能力。通过FACSAria III细胞分选仪从脂肪来源的MSCs中分离出Muse细胞，并对其多能性进行评估。分化后，对源自Muse细胞和非Muse细胞的IPCs进行胰岛素分泌评估。Muse细胞占MSCs的3.2±0.7%，约82%的Muse细胞抗阶段特异性胚胎抗原-3（SSEA-3）呈阳性。多能性标志物在Muse细胞中的表达高于非Muse细胞。通过流式细胞术分析，Muse细胞中产生的IPCs百分比更高。在共聚焦显微镜下，Muse细胞表达胰岛素和C肽，而非Muse细胞中未检测到。我们的研究结果表明，Muse细胞是一种新的成年多能亚群，能够产生更多数量的功能性IPCs。
