Hassan Amal Ahmed Mohamed, Balabel Esraa Aly, Oraby Hanaa Abdel Sadek, Darwish Samy Anwar
Cell Biology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 El Bohouth St., El Dokki, P.O. Box 12622, Giza, Egypt.
Animal Production Research Institute, 7 Nady EL Said St., El Dokki, P.O. Box 12618, Giza, Egypt.
J Genet Eng Biotechnol. 2018 Dec;16(2):499-505. doi: 10.1016/j.jgeb.2018.07.006. Epub 2018 Aug 9.
Enrichment of barcode databases with mitochondrial cytochrome oxidase subunit I (COI) barcode sequences in different animal taxa has become important for identification of animal source in food samples to prevent commercial fraud. In this study, COI barcode sequence in seventy one river buffalo samples were determined, analyzed and deposited in Genbank barcode database and barcode of life database (BOLD) to contribute for construction of public reference library for COI barcode sequence in river buffalo. Moreover COI barcode sequence was used to identify the closely related buffalo groups: river buffalo, swamp buffalo, lowland anoa and African buffalo. Results indicated the success of the COI barcode in the identification of each of the tested groups. Whereas a suggested sequence of other mitochondrial segment representing two successive transfer RNA (tRNA) genes; tRNA-Threonine (MT-TT) and tRNA-Proline (MT-TP) was failed to be used as a barcode marker for differentiation between the tested buffalo groups.
用不同动物类群的线粒体细胞色素氧化酶亚基I（COI）条形码序列丰富条形码数据库，对于识别食品样本中的动物来源以防止商业欺诈变得至关重要。在本研究中，测定、分析了71份河水牛样本的COI条形码序列，并将其存入Genbank条形码数据库和生命条形码数据库（BOLD），为构建河水牛COI条形码序列的公共参考文库做出贡献。此外，COI条形码序列被用于识别亲缘关系密切的水牛群体：河水牛、沼泽水牛、低地倭水牛和非洲水牛。结果表明COI条形码在识别每个测试群体方面取得了成功。而代表两个连续转运RNA（tRNA）基因的其他线粒体片段的建议序列；苏氨酸转运RNA（MT-TT）和脯氨酸转运RNA（MT-TP）未能用作区分测试水牛群体的条形码标记。
