Genetic variation in the first intron and exon of the gene in several Indonesian cattle populations.

BACKGROUND AND AIM

(), a member of the transforming growth factor-b family, is a negative regulator of muscle mass. This study aimed to detect the genetic variation of the 1160 bp fragment of exon 1 and part of intron 1 of the gene in several cattle populations raised in Indonesia.

MATERIALS AND METHODS

Polymerase chain reaction products of the gene amplified from 92 animals representing 10 cattle populations (Peranakan Ongole [PO], Belgian Blue x PO cross, Rambon, PO x Bali cross, Jabres, Galekan, Sragen, Donggala, Madura, and Bali) were sequenced, compared, and aligned with bovine of (GenBank Acc. No. AF320998.1) and (GenBank Acc. No. AY794986.1).

RESULTS

Four nucleotide substitutions (nt 1045 and 1066 in intron 1; nt 262 and 418 in exon 1) and two indels (nt 807 and 869 in intron 1) were synonymous mutations. Among these substitutions, only the nt 262G>C and nt 418A>G loci were polymorphic in all populations, except Bali cattle. The frequencies of the nt 262C (0.82) and nt 418A (0.65) alleles were highest. For the nt 262G>C locus, the CC genotype had the highest frequency (0.66) followed by GC (0.30) and CC (0.03). For the nt 418A>G locus, the AG genotype had the highest frequency (0.52) followed by AA (0.39) and GG (0.09).

CONCLUSION

The results, showing genetic variations in exon 1 and intron 1 of the gene, might be helpful for future association studies.