The Third Affiliated Hospital and School of Basic Medicine, Kunming Medical University, Kunming 650500, China.
Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Dis Markers. 2019 Jan 10;2019:4081962. doi: 10.1155/2019/4081962. eCollection 2019.
The main aim of the present study was to investigate the biological function of uric acid. The level of uric acid in different organs in normal male rats was determined with uric acid assay kits, and the expression level of genes in the organs was determined by RNA quantitative sequencing. The correlation analysis between uric acid in the organs and gene expression (measured by FPKM value) was made. Serum uric acid (SUA) in patients with breast cancer or with breast benign tumor was assayed when the diagnosis was made, and SUA in patients with breast cancer was also assayed just after chemotherapy. There were 1937 mRNAs whose expression level significantly correlated with the level of uric acid, and most of which were associated with purine or nucleoside metabolism, cellular metabolism, cell cycles, and cell death pathways. Further analysis showed that the level of uric acid was highly correlated with cell death rather than cell viability. The level of SUA in patients with breast cancer was higher than that in patients with breast benign tumor, and the SUA increased after chemotherapy. All the results suggested that uric acid was mainly synthesized from local nucleosides degraded from dead cells, and uric acid could be an important biomarker for cell death rather than an antioxidant for neural protection.
本研究的主要目的是探究尿酸的生物学功能。采用尿酸检测试剂盒测定正常雄性大鼠不同器官中的尿酸水平，并用 RNA 定量测序法测定器官中的基因表达水平。对器官中尿酸与基因表达（以 FPKM 值衡量）之间的相关性进行分析。在诊断时测定乳腺癌患者或乳腺良性肿瘤患者的血清尿酸（SUA），并在化疗后立即测定乳腺癌患者的 SUA。有 1937 个 mRNAs 的表达水平与尿酸水平显著相关，其中大部分与嘌呤或核苷代谢、细胞代谢、细胞周期和细胞死亡途径有关。进一步分析表明，尿酸水平与细胞死亡而非细胞活力高度相关。乳腺癌患者的 SUA 水平高于乳腺良性肿瘤患者，且化疗后 SUA 升高。所有结果表明，尿酸主要由来自死亡细胞的局部核苷降解产生，尿酸可能是细胞死亡的重要生物标志物，而非神经保护的抗氧化剂。
