Terada Noriko, Miyata Manabu, Muraoka Yuki, Hata Masayuki, Fujimoto Masahiro, Yokota Satoshi, Nakanishi Hideo, Suda Kenji, Yoshikawa Munemitsu, Ooto Sotaro, Ohtsuki Hiroshi, Tsujikawa Akitaka
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
J Ophthalmol. 2019 Jan 10;2019:2097087. doi: 10.1155/2019/2097087. eCollection 2019.
Several studies have indicated morphological changes in the choroid in amblyopia cases. This study investigates whether choroidal vasculature was different among amblyopic and fellow eyes in unilateral amblyopia patients and healthy eyes, using en face images acquired via swept-source optical coherence tomography (SS-OCT).
Prospective, observational case-control study.
This study included 14 consecutive patients with unilateral amblyopia and 22 age- and axial length-matched healthy eyes. Using SS-OCT, we obtained en face images of choroidal vasculature midway through the subfoveal inner and total choroid, corresponding to the vasculature of the choriocapillaris and Sattler's layer (inner choroid) and Haller's layer (outer choroid), respectively. We analyzed the en face images of the inner and outer choroidal vascular areas in 3 × 3 mm squares adjusted from 6 × 6 mm squares, using Littmann's magnification correction, after binarization of the images as a portion of the whole area.
The outer choroidal vascular areas were larger in both amblyopic and fellow eyes than in healthy eyes (both < 0.001), although there were no significant differences in inner (56.35 ± 2.46% and 56.27 ± 3.75%, respectively) or outer (61.49 ± 4.95% and 61.48 ± 3.73%, respectively) choroidal vascular area between amblyopic and fellow eyes (=0.98 and 0.91, respectively). An outer choroidal vascular area of 59% was set as an appropriate cutoff value for distinguishing patients from controls.
The outer choroidal vascular area was larger in both amblyopic eyes and fellow eyes compared to healthy eyes. Our findings may help clarify the etiology of amblyopia.
多项研究表明弱视患者脉络膜存在形态学改变。本研究利用扫频光学相干断层扫描（SS-OCT）获取的正面图像，调查单侧弱视患者的弱视眼、对侧眼以及健康对照眼的脉络膜血管系统是否存在差异。
前瞻性观察性病例对照研究。
本研究纳入了14例连续的单侧弱视患者以及22只年龄和眼轴长度匹配的健康对照眼。使用SS-OCT，我们获取了黄斑中心凹下脉络膜内层和全层中途的脉络膜血管正面图像，分别对应脉络膜毛细血管层和萨特勒层（脉络膜内层）以及哈勒层（脉络膜外层）的血管。在将图像二值化作为整个区域的一部分后，我们使用利特曼放大校正，对从6×6mm正方形调整而来的3×3mm正方形内的脉络膜内外层血管区域正面图像进行分析。
弱视眼和对侧眼的脉络膜外层血管区域均大于健康对照眼（均P<0.001），尽管弱视眼和对侧眼之间的脉络膜内层（分别为56.35±2.46%和56.27±3.75%）或外层（分别为61.49±4.95%和61.48±3.73%）血管区域无显著差异（分别为P=0.98和P=0.91）。将脉络膜外层血管区域59%设定为区分患者与对照的合适截断值。
与健康对照眼相比，弱视眼和对侧眼的脉络膜外层血管区域均更大。我们的研究结果可能有助于阐明弱视的病因。
