Department of Chemistry and Biochemistry , University of California Santa Barbara , Santa Barbara , California 93106 , United States.
Center for Integrated Nanotechnologies , Los Alamos National Laboratory , Los Alamos , New Mexico 87545 , United States.
Bioconjug Chem. 2019 Mar 20;30(3):853-860. doi: 10.1021/acs.bioconjchem.9b00004. Epub 2019 Feb 20.
Plasmon-resonant nanoparticles provide unprecedented spatiotemporal control over the release of diverse cargoes into cells. Here we compare the loading, release and internalization efficiencies, and effectiveness of post transcriptional gene silencing of hollow gold nanoshells, hollow gold nanocages, and gold nanorods with plasmons tuned to absorb near-infrared light at 800 nm. The hollow gold nanoshells can be loaded with up to three times more siRNA cargo compared to nanocages and nanorods; however, nanorods exhibit the highest efficiency of release of attached siRNA strands when exposed to pulsed 800 nm laser excitation. In cellular treatments, all particles demonstrated efficient internalization into HeLa cells, but the nanoshells and nanocages display the highest downregulation of GFP expression 72 h after treatment. These results provide novel insights into the relative efficiencies of three structurally distinct types of gold nanoparticles as siRNA carriers and we examine different parameters that may influence their efficacy.
等离子体共振纳米粒子为将各种货物递送到细胞内提供了前所未有的时空控制。在这里，我们比较了负载、释放和内化效率，以及近红外 800nm 光吸收等离子体调谐的中空金纳米壳、中空金纳米笼和金纳米棒在后转录基因沉默中的效果。与纳米笼和纳米棒相比，中空金纳米壳可负载多达三倍的 siRNA 货物；然而，当用脉冲 800nm 激光激发时，附着的 siRNA 链的释放效率最高。在细胞处理中，所有颗粒都能有效地进入 HeLa 细胞，但纳米壳和纳米笼在治疗后 72 小时内显示出最高的 GFP 表达下调。这些结果为三种结构不同的金纳米粒子作为 siRNA 载体的相对效率提供了新的见解，并研究了可能影响其功效的不同参数。
