Ryzhmanova Yana, Abashina Tatyana, Petrova Daria, Shcherbakova Viktoria
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki 3, Pushchino, Moscow, 142290, Russian Federation.
Gubkin Russian State University of Oil and Gas, 65/1 Leninsky Prospekt, Moscow, 119991, Russian Federation.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1081-1086. doi: 10.1099/ijsem.0.003272. Epub 2019 Feb 8.
A psychrotolerant non-spore-forming sulfate-reducing bacterium, strain K3S, was isolated from a Yamal Peninsula cryopeg within permafrost. Strain K3S grew at subzero temperatures and required Na for growth. The new bacterium was able to use lactate, formate, pyruvate, fumarate, alanine, ethanol and molecular hydrogen as electron donors in the presence of sulfate, and used sulfate, sulfite, thiosulfate and elemental sulfur as electron acceptors in the presence of lactate. Fe(III)-citrate and Fe(III)-EDTA were reduced without visible growth. Major polar lipids were рhosphatidylserine, рhosphatidylethanolamine, phospholipids, cardiolipin and aminolipid; major cellular fatty acids were C16 : 1ω7, C16 : 0 and C18 : 1ω7; and the predominant isoprenoid quinone was MK-6 (H2). The genomic DNA G+C content was found to be 42.33 mol%. Phylogenetic analysis showed that the closest relative of the new isolate was Desulfovibrio ferrireducens strain 61 with 97.1 % 16S rRNA gene similarity. In addition, the ANI value between strain K3S and D. ferrireducens 61 was 82.1 %. On the basis of the genomic and polyphasic taxonomy data of strain K3S, we conclude that the strain is a representative of a novel species Desulfovibrio gilichinskyi sp. nov. (=VKM B-2877=DSM 100341).
从亚马尔半岛多年冻土内的低温冻土中分离出一株耐冷的、不产芽孢的硫酸盐还原菌K3S菌株。K3S菌株能在零下温度下生长，且生长需要钠。这种新细菌在有硫酸盐存在的情况下，能够利用乳酸、甲酸、丙酮酸、富马酸、丙氨酸、乙醇和分子氢作为电子供体，并在有乳酸存在的情况下，利用硫酸盐、亚硫酸盐、硫代硫酸盐和元素硫作为电子受体。在没有明显生长的情况下，柠檬酸铁（III）和乙二胺四乙酸铁（III）被还原。主要极性脂类为磷脂酰丝氨酸、磷脂酰乙醇胺、磷脂、心磷脂和氨基脂；主要细胞脂肪酸为C16 : 1ω7、C16 : 0和C18 : 1ω7；主要类异戊二烯醌为MK-6（H2）。发现基因组DNA的G+C含量为42.33摩尔%。系统发育分析表明，新分离菌株的最亲近亲缘种是铁还原脱硫弧菌61菌株，16S rRNA基因相似性为97.1%。此外，K3S菌株与铁还原脱硫弧菌61之间的ANI值为82.1%。基于K3S菌株的基因组和多相分类学数据，我们得出结论，该菌株是吉尔钦斯基脱硫弧菌新种（=VKM B-2877=DSM 100341）的代表菌株。
