Gerlinger-Romero Frederico, Addinsall Alex B, Lovering Richard M, Foletta Victoria C, van der Poel Chris, Della-Gatta Paul A, Russell Aaron P
School of Exercise and Nutrition Sciences, Deakin University;
Centre for Molecular and Medical Research, School of Medicine, Deakin University.
J Vis Exp. 2019 Jan 17(143). doi: 10.3791/58696.
Assessment of skeletal muscle contractile function is an important measurement for both clinical and research purposes. Numerous conditions can negatively affect skeletal muscle. This can result in a loss of muscle mass (atrophy) and/or loss of muscle quality (reduced force per unit of muscle mass), both of which are prevalent in chronic disease, muscle-specific disease, immobilization, and aging (sarcopenia). Skeletal muscle function in animals can be evaluated by a range of different tests. All tests have limitations related to the physiological testing environment, and the selection of a specific test often depends on the nature of the experiments. Here, we describe an in vivo, non-invasive technique involving a helpful and easy assessment of force frequency-curve (FFC) in mice that can be performed on the same animal over time. This permits monitoring of disease progression and/or efficacy of a potential therapeutic treatment.
评估骨骼肌收缩功能对于临床和研究目的而言都是一项重要的测量。许多情况会对骨骼肌产生负面影响。这可能导致肌肉质量丧失（萎缩）和/或肌肉质量下降（每单位肌肉质量的力量降低），这两种情况在慢性疾病、肌肉特异性疾病、制动和衰老（肌肉减少症）中都很常见。动物的骨骼肌功能可以通过一系列不同的测试来评估。所有测试都存在与生理测试环境相关的局限性，特定测试的选择通常取决于实验的性质。在此，我们描述一种体内非侵入性技术，该技术涉及对小鼠力量频率曲线（FFC）进行有益且简便的评估，并且可以在同一动物上随时间进行。这允许监测疾病进展和/或潜在治疗方法的疗效。
