Department of Athletic Training and Clinical Nutrition, University of Kentucky; Center for Muscle Biology, University of Kentucky.
Department of Physical Therapy, East Carolina University.
J Vis Exp. 2021 Mar 4(169). doi: 10.3791/62211.
Skeletal muscle plasticity in response to countless conditions and stimuli mediates concurrent functional adaptation, both negative and positive. In the clinic and the research laboratory, maximal muscular strength is widely measured longitudinally in humans, with knee extensor musculature the most reported functional outcome. Pathology of the knee extensor muscle complex is well documented in aging, orthopedic injury, disease, and disuse; knee extensor strength is closely related to functional capacity and injury risk, underscoring the importance of reliable measurement of knee extensor strength. Repeatable, in vivo assessment of knee extensor strength in pre-clinical rodent studies offers valuable functional endpoints for studies exploring osteoarthritis or knee injury. We report an in vivo and non-invasive protocol to repeatedly measure isometric peak tetanic torque of the knee extensors in mice across time. We demonstrate consistency using this novel method to measure knee extensor strength with repeated assessment in multiple mice producing similar results.
骨骼肌对无数条件和刺激的可塑性介导了同时发生的功能适应，既有负面的也有正面的。在临床和研究实验室中，人类的最大肌肉力量被广泛地进行纵向测量，其中膝关节伸肌的功能结果报告最多。膝关节伸肌复合体的病理学在衰老、骨科损伤、疾病和废用中得到了充分的记录；膝关节伸肌力量与功能能力和受伤风险密切相关，突出了可靠测量膝关节伸肌力量的重要性。在临床前啮齿动物研究中，可重复的、体内评估膝关节伸肌力量为探索骨关节炎或膝关节损伤的研究提供了有价值的功能终点。我们报告了一种在体内和非侵入性的方案，用于在时间上反复测量小鼠膝关节伸肌的等长最大强直扭矩。我们使用这种新方法证明了一致性，该方法在多次评估中用于测量膝关节伸肌力量，在多只小鼠中产生了相似的结果。
